Review article
https://doi.org/10.37797/ig.41.2.4
„Non-culture" methods in diagnostics of invasive candidiasis
Dea Ratković
; Služba za mikrobiologiju, Nastavni zavod za javno zdravstvo Istarske županije, Pula, Hrvatska
Maja Abram
; Zavod za mikrobiologiju i parazitologiju, Medicinski fakultet, Sveučilište u Rijeci, Rijeka, Hrvatska; Klinički zavod za kliničku mikrobiologiju, Klinički bolnički centar Rijeka, Rijeka, Hrvatska
Marina Bubonja-Šonje
; Zavod za mikrobiologiju i parazitologiju, Medicinski fakultet, Sveučilište u Rijeci, Rijeka, Hrvatska; Klinički zavod za kliničku mikrobiologiju, Klinički bolnički centar Rijeka, Rijeka, Hrvatska
Abstract
Invasive fungal infections, especially those caused by Candida species, are not uncommon in critically ill patients and are associated with significant morbidity and mortality. Early diagnosis is essential because delaying the use of antifungal drugs can affect treatment outcome. Due to the limitations of classical culture-based methods (they are time-consuming and insensitive), trends in diagnostics switch to “non-culture” based methods for early diagnosis of invasive candidiasis. „Non-culture" methods, such as biomarker detection or Polymerase Chain Reaction (PCR) techniques, have great diagnostic potential. For example, a negative PCR or 1,3-β-D-glucan (BDG) test result have a high negative predictive value (NPV). In order to increase the reliability of each method, it is recommended to combine different methods as well as to interpret the obtained results in accordance with the clinical, epidemiological, laboratory and other patient data.
Keywords
β-D-glucan; Candida; diagnosis; invasive candidiasis; mannan; Polymerase Chain Reaction
Hrčak ID:
270533
URI
Publication date:
10.1.2022.
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