Original scientific paper

https://doi.org/10.2478/v10007-008-0017-0

Development and validation of spectrophotometric methods for determination of ceftazidime in pharmaceutical dosage forms

BASAVARAJ HIREMATH ; Department of Chemistry, Gulbarga University, Gulbarga-585 106, India
BENNIKALLU HIRE MATHADA MRUTHYUNJAYASWAMY ; Department of Chemistry, Gulbarga University, Gulbarga-585 106, India

Abstract

Two spectrophotometric methods for the determination of ceftazidime (CFZM) in either pure form or in its pharmaceutical formulations are described. The first method is based on the reaction of 3-methylbenzothiazolin-2-one hydrazone (MBTH) with ceftazidime in the presence of ferric chloride in acidic medium. The resulting blue complex absorbs at λmax 628 nm. The second method describes the reaction between the diazotized drug and N-(1-naphthyl)ethylenediamine dihydrochloride (NEDA) to yield a purple colored product with λmax at 567 nm. The reaction conditions were optimized to obtain maximum color intensity. The absorbance was found to increase linearly with increasing the concentration of CFZM; the systems obeyed the Beer’s law in the range 210 and 1050 µg mL1 for MBTH and NEDA methods, respectively. LOD, LOQ and correlation coefficient values were 0.15, 0.79 and 0.50, 2.61. No interference was observed from common excipients present in pharmaceutical formulations. The proposed methods are simple, sensitive, accurate and suitable for quality control applications.

Keywords

ceftazidime; diazotization; spectrophotometry; pharmaceutical formulation

Hrčak ID:

25030

URI

https://hrcak.srce.hr/25030

Publication date:

1.9.2008.

Article data in other languages: croatian

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