Original scientific paper
https://doi.org/10.5599/jese.1231
A sensitive Cu(salophen) modified screen-printed electrode for simultaneous determination of dopamine and uric acid
Hadi Beitollahi
; School of Medicine, Bam University of Medical Sciences,Bam, Iran
Somayeh Tajik
; Research Center of Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran
Mohammad Reza Aflatoonian
; Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran
Asghar Makarem
; Department of Rehabmanagement, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran
Abstract
This research applied a nanostructured electrochemical sensor with a screen-printed electrode (SPE) for examining the dopamine (DA) electrocatalytic oxidation when uric acid (UA) was present. Cu(salophen) nanostructured modified SPE (Cu(salophen)/SPE)was employed to investigate the electrochemical behavior of DA. At optimal pH (pH7.0), oxidation of DA at the modified electrode takes place at a potential around 100 mV less positive than at the unmodified SPE. Chronoamperometry was used to determine the diffusion coefficient of DA (D=1.96×10-5cm2s-1). Differential pulse voltammetry (DPV) showed linear response in the range between 0.2-450.0 μM for DA. The limit of detection (LOD) of DA was computed to be 0.05 μM. Moreover, Cu(salophen)/SPE was employed for determining DA in the presence of UA using DPV. The DPV results showed that at the modified electrode, two well-separated oxidation peaks of DA and UA could be obtained at potentials of 180 and 325 mV, respectively. This separation forms the basis for the co-detection of these two materials on the surface of Cu(salophen)/SPE. This sensor was then employed to determine DA and UA in real specimens.
Keywords
Dopamine; uric acid; screen printed electrode; voltammetry, chemically modified electrodes, real sample analysis
Hrčak ID:
273221
URI
Publication date:
24.2.2022.
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