Original scientific paper

Influence of adjuvants Saponin and Montanide ISA 50 on immune response of calves immunized with purified midgut antigen of Hyalomma marginatum isaaci

Gurusamy Ponnudurai ; Department of Veterinary Parasitology, Veterinary College and Research Institute, Namakkal, Tamil Nadu, India
Lalitha John ; Department of Veterinary Parasitology, Madras Veterinary College, Chennai
Tirunelveli Jayagopal Harikrishnan ; Department of Veterinary Parasitology, Veterinary College and Research Institute, Namakkal, Tamil Nadu, India

Abstract

Immune status of calves, immunized with purified midgut antigen of Hyalomma marginatum isaaci, was assessed by standard immunological tests. Leukocyte migration inhibition test showed inhibition from day 21 post-infection in calves that received midgut antigen with Saponin as adjuvant (Saponin group) while significant inhibition was observed from 28 DPI in the Montanide group. Intradermal test revealed immediate hypersensitivity reaction within 15 m. and the highest skin fold thickness was observed after 24 h. of inoculation of antigen (14.50 ± 1.50 mm in the Saponin group and 12.00 ± 0.70 mm in the Montanide group). Similarly, a significant humoral response as judged by ELISA was observed from 7 DPI (P<0.01) in the Saponin group, with a peak value on 42 DPI, whereas the significant antibody was observed only on 21 DPI, with a peak value on 30 DPI in the Montanide group.

Keywords

immune response; Hyalomma marginatum isaaci; midgut antigen; calves

Hrčak ID:

25174

URI

https://hrcak.srce.hr/25174

Publication date:

20.2.2007.

Article data in other languages: croatian

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