Original scientific paper

https://doi.org/10.24099/vet.arhiv.1390

Identification of monomorphic and polymorphic genes associated with recessive fertility defects in Holstein cows reared in Kazakhstan

Yessengali Ussenbekov ; Department of Obstetrics, Surgery and Biotechnology of Animal Reproduction, Kazakh National Agrarian Research University, Almaty, Kazakhstan
Aigerim Bagdat ; Department of Obstetrics, Surgery and Biotechnology of Animal Reproduction, Kazakh National Agrarian Research University, Almaty, Kazakhstan
Zhanat Bimenova ; Department of Obstetrics, Surgery and Biotechnology of Animal Reproduction, Kazakh National Agrarian Research University, Almaty, Kazakhstan
Kanat Orynkhanov ; Department of Obstetrics, Surgery and Biotechnology of Animal Reproduction, Kazakh National Agrarian Research University, Almaty, Kazakhstan
Przemysław Sobiech ; Department of Internal Medicine with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland
Marko Samardžija ; Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Chandra S. Pareek ; Department of Basic Science and Pre-clinics, Institute of Veterinary Medicine, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University, ul. Gagarina 7, 87-100 Toruń, Poland
Attila Dobos ; CEVA-Phylaxia Co Ltd., Budapest, Hungary

Abstract

Haplotypes of candidate genes namely: apoptotic protease activating factor 1 (APAF1 p.Q579X or HH1),
glycinamide ribonucleotide formyltransferase (GART or HH4), structural maintenance of chromosomes 2 (SMC2
or HH3), and haplotype cholesterol deficiency (HCD) genes associated with recessive fertility defects (loss of
fertility) were investigated in imported Canadian Holstein cows reared at “Medeu Commerce” LLP breeding farm in
Kazakhstan. The genotypic profiling of the APAF1/HH1, GART/HH4 fertility haplotype carriers was carried out by
PCR-RFLP methods using BstC8I and Tru9I and MseI, while the genotypic profiling of the SMC2/HH3, and HCD
fertility haplotype carriers was carried out using our own primer designed by internal primer marker methods. The
study revealed that the PCR-RFLP diagnostic markers APAF1/HH1 and GART/HH4 for recessive fertility defects
were monomorphic in the Canadian Holstein cows investigated. However, the diagnostic markers SMC2/HH3 and
HCD fertility haplotype carriers (our own design diagnostic markers) were polymorphic, with frequencies of 3%
and 11%, respectively, in the investigated Canadian Holstein cows. The study concluded that genetic monitoring of
recessive fertility defects enables the timely identification of carriers of harmful lethal mutations, and control of the
fertility haplotype elimination process.

Keywords

loss of fertility; mutations; SNP; genetic defects; haplotypes; PCR-RFLP; APAF1; HH1; SMC2; HH3; GART; HH4; HCD; APOB; Holstein cow

Hrčak ID:

274810

URI

https://hrcak.srce.hr/274810

Publication date:

24.3.2022.

Article data in other languages: croatian

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