Original scientific paper

https://doi.org/10.24099/vet.arhiv.1420

The effects of resveratrol on SIRT2, SIRT3 expression levels and oxidative DNA damage in fumonisin-induced hepatotoxicity in BALB/c mice

Canan Gulmez ; Department of Pharmacy Services, Tuzluca Vocational High School, Igdir University, Igdir, Turkey
Rıza Yalcin ; Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, Turkey
Asım Kart ; Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, Turkey
Emine Atakisi ; Department of Biochemistry, Faculty of Veterinary Medicine, Kafkas University, Kars, Turkey
Moldaliev Z. Tumakovich ; Department of Biology Medical Institute Osh State University, Osh, Kyrgyzstan
Onur Atakisi ; Department of Chemistry, Faculty Science and Letter, Kafkas University, Kars, Turkey

Abstract

Oxidative stress, which is characterized by disruption of the oxidant/antioxidant balance, causes pathological
processes, including toxicities induced by certain mycotoxins. The present study was designed to investigate the
effects of resveratrol on sirtuin deacetylases (SIRT2 and SIRT3), nitric oxide (NO), reduced glutathione (GSH) and
malondialdehyde (MDA) in fumonisin B1-induced hepatotoxicity. Regarding the experimental design, forty BALB/c
mice were divided into four groups corresponding to the control, resveratrol (10 mg/kg, i.p), fumonisin B1 (2.25 mg/
kg, i.p) and resveratrol + fumonisin B1 (10 mg/kg + 2.25 mg/kg) groups. At the end of the 14 day-treatment, expression
levels of SIRT2 and SIRT3 protein in the serum and liver were revealed by western blotting and antioxidant/oxidant
activity analysis. SIRT2 and SIRT3 expression levels in the liver were significantly decreased by fumonisin B1 in
comparison to the control. However, resveratrol supplementation coupled with fumonisin B1 increased the expression
levels of SIRT2 and SIRT3, in relation to the fumonisin B1 treatments alone, but did not exhibit significant differences
from those of the control group. As substantial indicators of stress and damage, the 8-OH-2-deoxyguanosine, NO
and MDA levels of the liver tissue were assayed, and were higher in the fumonisin B1-treated groups, in relation to
the control. As expected, resveratrol treatment significantly reduced the levels of NO and MDA in comparison to the
fumonisin B1 treatments alone. Also, resveratrol attenuated the liver 8-OH-2- deoxyguanosine levels in the resveratrol
+ fumonisin B1 group. In conclusion, the findings revealed that resveratrol might possess protective effects against
fumonisin-induced hepatotoxicity through modulation of the expression of sirtuin proteins, and by protecting the cell
from oxidative/nitrosative stress.

Keywords

mycotoxins; hepatoprotection; dietary phenolic compounds; sirtuins; nitric oxide; lipid peroxidation

Hrčak ID:

274816

URI

https://hrcak.srce.hr/274816

Publication date:

24.3.2022.

Article data in other languages: croatian

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