Acta clinica Croatica, Vol. 60. No. 4., 2021.
Preliminary communication
https://doi.org/10.20471/acc.2021.60.04.25
Can IgG Antibodies Directed against Different Targets Solve the Problem of False-Positive Results in SARS-CoV-2 Serology?
Nataša Beader
orcid.org/0000-0003-3692-5606
; Department of Clinical and Molecular Microbiology, Zagreb University Hospital Center, Zagreb, Croatia; School of Medicine, University of Zagreb, Zagreb, Croatia
Ivana Ivana Mareković
; Department of Clinical and Molecular Microbiology, Zagreb University Hospital Center, Zagreb, Croatia; School of Medicine, University of Zagreb, Zagreb, Croatia
Abstract
In addition to RT-PCR assays, serology testing that has been recognized as a useful
tool to assess the spread of infection in the population is considered successful and important
strategy in the control of the global pandemic of SARS-CoV-2 infection. Now, a great number of
manufacturers offer their serologic tests on the market. When interpretating the results, the rate of
seroprevalence should be taken in consideration because it may influence the positive predictive value,
as well as cross-reactivity with other coronaviruses in case of assays with poorly designed antigens. We
present results of 11 patients with different clinical background and tested with two different serologic
tests, DIAPRO (ELISA; Sesto San Giovanni, Italy) and VIDAS (ELFA; BioMerieux, Marcy
I’Etoile, France). The results obtained by the former test showed ten of these patients to be IgG positive
and one patient was IgG weakly positive with different confidence index. The latter test discriminated
positive results with medium confidence index on the former test as negative. The results obtained
with two serology tests were concordant with the observation that the results with medium
confidence index may indicate cross-reactivity.
Keywords
SARS-CoV 2; Serology; Anti- SARS-CoV-2 IgG; False-positive results; ELISA; ELFA
Hrčak ID:
275552
URI
Publication date:
1.12.2021.
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