Veterinary Archives, Vol. 92 No. 2, 2022.
Original scientific paper
https://doi.org/10.24099/vet.arhiv.1028
Epidemiology of bovine viral diarrhea virus infection on a dairy farm - clinical signs, seroprevalence, virus detection and genotyping
Nada Kučer
; Clinic of Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Evica Marković
; Ministry of Agriculture, Zagreb, Croatia
Jelena Prpić
; Department of Virology, Croatian Veterinary Institute, Zagreb, Croatia
Damir Rudan
; Ministry of Agriculture, Zagreb, Croatia
Lorena Jemeršić
; Department of Virology, Croatian Veterinary Institute, Zagreb, Croatia
Zvonimir Hajek
; Farm of dairy cows, Feričanci, Croatia
Kristijan Škaro
; Department of Microbiology and Infectious Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Marina Pavlak
; Department of Veterinary Economics and Analytical Epidemiology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Nevenka Rudan
; Department of Microbiology and Infectious Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Abstract
In this study we investigated the presence of Bovine Viral Diarrhoea Virus (BVDV) antibodies and antigens in a dairy cow herd that had reported clinical signs related to BVDV infection. The aim of the study was to confirm BVDV infection on the dairy farm with 1500 Holstein-Friesian cattles, and genetically analyse the BVDV type/s circulating in the herd. Serum samples (n=233) were collected from clinically infected cattles and tested for the presence of BVDV antibodies and antigens. The virus neutralization test showed that 194 out of 233 sera of the tested cows were positive (83.26%), and by Ab-ELISA 203 sera samples of the tested cows were shown to be positive (87.12%). The presence of antigens was proven with the Ag-ELISA test in 2 out of 233 samples (0.86%). After RT-PCR and phylogenetic analysis of two BVD virus isolates, it was found that both isolates belonged to genotype 1, subtype BVDV-1d. This is the first time that the BVDV-1d subtype has been confirmed in Croatia, although the BVDV-1b and BVDV-1f subtypes have been detected previously on Croatian dairy farms.
Keywords
BVDV; VNT; At-ELISA; Ag-ELISA; RT-PCR; sequencing; phylogenetic analysis; BVDV 1 subtype 1d
Hrčak ID:
277976
URI
Publication date:
19.5.2022.
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