Original scientific paper
https://doi.org/10.5513/JCEA01/23.2.3440
The effect of additives on mycotoxic contamination of maize silages
Mária KALÚZOVÁ
; Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Institute of Nutrition and Genomics, Nitra, Slovakia
Miroslav JURÁČEK
orcid.org/0000-0003-1260-2319
; Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Institute of Nutrition and Genomics, Nitra, Slovakia
Daniel BÍRO
; Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Institute of Nutrition and Genomics, Nitra, Slovakia
Branislav GÁLIK
; Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Institute of Nutrition and Genomics, Nitra, Slovakia
Milan ŠIMKO
; Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Institute of Nutrition and Genomics, Nitra, Slovakia
Michal ROLINEC
; Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Institute of Nutrition and Genomics, Nitra, Slovakia
Ondrej HANUŠOVSKÝ
; Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Institute of Nutrition and Genomics, Nitra, Slovakia
Eva MIXTAJOVÁ
; Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Institute of Nutrition and Genomics, Nitra, Slovakia
Renata KOLLÁTHOVÁ
; Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Institute of Nutrition and Genomics, Nitra, Slovakia
Stanislava DROTÁROVÁ
; Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Institute of Nutrition and Genomics, Nitra, Slovakia
Abstract
Addition of silage additives is commonly concentrated on the improvement of nutritive and fermentative features of silages, but in recent years, mycotoxic contamination is also a feature monitored after the treatment. This study aimed to evaluate the effect of silage additives, in the concrete inoculant consisting of a mixture of Lactobacillus buchneri LN40177, Lactobacillus casei LC329090 and urea, on the concentration of major mycotoxins in maize silages. The ensilage mass was made in three variants, which consisted of maize silage (control variant C), maize silage with the addition of a commercial inoculant (variant A) and maize silage with the addition of urea (variant U). The commercial inoculant was added to the cut mass at a dose of 1 g/t of mass and urea at a dose of 5 kg/t of mass. After application of silage additives, the mass was stored and sealed in silage units. Mycotoxin analysis was performed using Veratox tests with ELISA reader, whereas average samples were prepared according to established protocols. The immuno-enzymatic method revealed that all samples of maize silage showed 100% contamination. The maize silages were characterized by the highest concentration of deoxynivalenol regardless of the treatment. The results confirmed the effect of silage additives on mycotoxic contamination of maize silages. Compared to the control variant, the commercial inoculant had negative increasing effect (P<0.05) on deoxynivalenol, ochratoxins and zearalenone, and a positive decreasing effect (P<0.05) on T-2 toxin and fumonisins. The urea addition resulted in significant reduction of T-2 toxin and increase of zearalenone.
Keywords
maize silage; inoculant, urea; mycotoxins
Hrčak ID:
279686
URI
Publication date:
27.6.2022.
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