Original scientific paper

https://doi.org/10.5513/JCEA01/23.2.3437

Chlorophyll a fluorescence as tool in breeding drought stress-tolerant soybean

Maja MATOŠA KOČAR orcid.org/0000-0002-3491-050X ; Agricultural Institute Osijek, Južno predgrađe 17, 31103 Osijek, Croatia
Ana JOSIPOVIĆ ; Independent researcher; Vukovar, Croatia
Aleksandra SUDARIĆ ; Center of Excellence for Biodiversity and Molecular Plant Breeding, University of Zagreb, Faculty of Agriculture, Svetošimunska cesta 25, 10000 Zagreb, Croatia
Tomislav DUVNJAK orcid.org/0000-0003-1545-1974 ; Agricultural Institute Osijek, Južno predgrađe 17, 31103 Osijek, Croatia
Marija VILJEVAC VULETIĆ orcid.org/0000-0001-8792-641X ; Agricultural Institute Osijek, Južno predgrađe 17, 31103 Osijek, Croatia
Monika MARKOVIĆ orcid.org/0000-0002-8742-6922 ; Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, Vladimira Preloga 1, 31000 Osijek, Croatia
Antonela MARKULJ KULUNDŽIĆ orcid.org/0000-0002-3181-8265 ; Agricultural Institute Osijek, Južno predgrađe 17, 31103 Osijek, Croatia

Abstract

Mitigating the effects of changing climate with adaptable cultivars while reducing the input burden of additional selection criteria is becoming a priority, especially as water shortage is limiting soybean production in Europe. To evaluate the functionality of the photosynthetic apparatus in drought, chlorophyll a (Chl a) fluorescence was measured in 16 elite soybean lines in drought and conditions with sufficient water supply in V2 (second node), R1 (beginning bloom), R4 (full pod), R5 (beginning seed) and R6 (full seed) stages. Developmental stage was a significant source of variation for all parameters, and PItotal was chosen as the most sensitive parameter in detecting the average drought effect. Genotypes G5, G9, and G10 had superior overall functioning of the photosynthetic apparatus in drought, while the photosynthetic apparatus of G12 and G16 was the least functional. The drought effect was determined to be the most relevant in R1, R4 and R6. G14 had highest PItotal in drought conditions in R1 and R4, while G9 had the highest drought-stressed PItotal in R6. G7 had the lowest drought-stressed PItotal in R1, G4 and G6 had the lowest in R4, and G8 had the lowest in R6. PItotal proved useful in breeding for abiotic stress tolerance, especially for excluding the material with the poorest photosynthetic apparatus function, which increases the efficiency of the selection process when a large number of genotypes needs to be screened. However, genotypes with superior photosynthetic apparatus functioning should be further tested in yield trials to confirm their drought tolerance and value for use in drought conditions.

Keywords

drought susceptibility; photosynthesis; photosynthetic efficiency; stages of development

Hrčak ID:

279690

URI

https://hrcak.srce.hr/279690

Publication date:

27.6.2022.

Article data in other languages: croatian

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