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Periodicum biologorum, Vol. 123 No. 3-4, 2021.

Original scientific paper

https://doi.org/10.18054/pb.v123i3-4.19206

Ligustrum vulgare leaves and fruit extracts induce apoptosis of human leukemia cells

Milan Zaric ; University of Kragujevac, Faculty of Medical Sciences, Department of Biochemistry, Kragujevac, Serbia
Suzana Popovic ; University of Kragujevac, Faculty of Medical Sciences, Centre for Molecular Medicine and Stem Cell Research, Kragujevac, Serbia
Dejan Baskic orcid id orcid.org/0000-0002-6152-0742 ; University of Kragujevac, Faculty of Medical Sciences, Centre for Molecular Medicine and Stem Cell Research, Kragujevac, Serbia
Danijela Jovanovic ; University of Kragujevac, Faculty of Medical Sciences, Department of Internal Medicine, Kragujevac, Serbia
Predrag Djurdjevic ; University of Kragujevac, Faculty of Medical Sciences, Department of Internal Medicine, Kragujevac, Serbia
Radica Zivkovic Zaric ; University of Kragujevac, Faculty of Medical Sciences, Department of Pharmacology and Toxicology, Kragujevac, Serbia
Petar Canovic ; University of Kragujevac, Faculty of Medical Sciences, Department of Biochemistry, Kragujevac, Serbia
Ivanka Zelen ; University of Kragujevac, Faculty of Medical Sciences, Department of Biochemistry, Kragujevac, Serbia

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Abstract

Background and purpose: Cytotoxic effects of Ligustrum vulgare leaves on HeLa cervical tumor cells suggested that Ligustrum vulgare extracts should be investigated as potential anticancer agents. Therefore, we examined a potential antileukemic activity of methanolic extracts of Ligustrum vulgare leaves and fruit extracts on two types of leukemia cells, MOLT-4 and JVM-13, lymphocytes isolated from the blood of 33 chronic lymphocytic leukemia (CLL) patients and on mononuclear leukocytes isolated from the blood of 18 healthy individuals.Material and methods: The cytotoxicity of examined extracts was measured by MTT assay and LDH activity test. The antiapoptotic potential of tested extracts was measured by Annexin V/7AAD flowcytometric assay.Results: The results showed that both extracts exhibited a moderate cytotoxic effect on all three types of leukemia cells. The Ligustrum vulgare leaf extract was the most effective on MOLT-4 cells, the fruit extract on JVM-13 cells and both extracts were equally effective on CLL cells. In addition, none of the tested extracts was toxic to healthy mononuclear cells. Both extracts acted by inducing apoptosis of leukemic cells.Conclusion: Ligustrum vulgare extracts exhibit significant antileukemic potential and should be further investigated.

Keywords

Ligustrum vulgare; apoptosis; chronic lymphocytic leukemia

Hrčak ID:

279954

URI

https://hrcak.srce.hr/279954

Publication date:

30.5.2022.

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