Original scientific paper
Expression of bone morphogenetic protein -2 and -7 during experimental inflammatory bowel disease
Ivana Marić
orcid.org/0000-0001-9318-5726
; Department of anatomy, School of medicine, University of Rijeka, Croatia
Natalia Kučić
; Department of physiology and immunology, School of medicine, University of Rijeka, Croatia
Blaženka Grahovac
; Department of pathology and pathoanatomy, School of medicine, University of Rijeka, Croatia
Dragica Bobinac
; Department of anatomy, School of medicine, University of Rijeka, Croatia
Slobodan Vukičević
; Department of anatomy, School of medicine, University of Zagreb, Croatia
Abstract
ABSTRACT. Aim: Bone morphogenetic proteins -2 and -7, along with their osteoinductive
function, play important role in the development and healing of different non-skeletal tissues
and organs. The aim of this study was to reveal the expression of BMP-2 and BMP-7 during
experimental inflammatory bowel disease, as well as after 14th day of BMP-7 treatment.
Methods: Experimental colitis was induced by the intracolonic administration of the clysma
containing 2,4,6-trinitrobenzensulphonic acid (TNBS) and ethanol. We investigated the
macroscopic and microscopic colon tissue damage during acute and chronic phases of colitis as
well as after IV treatment of BMP-7. Results: Imunohistochemical and RT-PCR analyses showed
expression of BMP-2 and -7 during acute and chronic phases of inflammatory bowel disease. In
the acute phase of colitis, BMP-2 and -7 expressions were located in mucosa of ulcer region. In
the chronic phase BMP-7 expression was placed in the colonocytes along the crypt, while BMP-2
expression was located in the coloncytes of surface epithelium and intercrypt table. BMP-7
treatment reduced the expression of BMP-7 and mRNA for BMP-7 but also increased the
expression of BMP-2 and mRNA for BMP-2 in colonic tissue. Conclusions: Systemic administration
of bone morphogenetic protein 7 decreased macroscopic and microscopic colonic tissue damage
during experimental inflammatory bowel disease. The expression pattern of BMP-2 and -7 during
inflammatory bowel disease, as well as after BMP-7 treatment suggests an important role of
bone morphogenetic proteins in control of colon tissue damage and healing during disease.
Keywords
bone morphogenetic protein-2; bone morphogenetic protein -7; immunohistochemistry; inflammatory bowel disease; polymerase chain reaction
Hrčak ID:
25952
URI
Publication date:
1.6.2008.
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