Veterinary Archives, Vol. 92 No. 4, 2022.
Original scientific paper
https://doi.org/10.24099/vet. arhiv.1300
Characterization of somatic and metabolic antigens of Cysticercus cellulosae and determination of immunodominant proteins
Kaushik Satyaprakash
; Department of Veterinary Public Health and Epidemiology, Nagpur Veterinary College, Nagpur, Maharashtra, India; Department of Veterinary Public Health and Epidemiology, Faculty of Veterinary and Animal Sciences, RGSC (Banaras Hindu University), Mirzapur, Uttar Pradesh, India
Wiqar Ahmed Khan
; Department of Veterinary Public Health and Epidemiology, Nagpur Veterinary College, Nagpur, Maharashtra, India
Nandkishor Namdeorao Zade
; Department of Veterinary Public Health and Epidemiology, Nagpur Veterinary College, Nagpur, Maharashtra, India
Sandeep Prabhakarrao Chaudhari
; Department of Veterinary Public Health and Epidemiology, Nagpur Veterinary College, Nagpur, Maharashtra, India
Shilpshri Vasant Shinde
; Department of Veterinary Public Health and Epidemiology, Nagpur Veterinary College, Nagpur, Maharashtra, India
Abstract
The present study shows the characterization of four somatic antigens (Whole Cyst Antigen (WCA), Cystic Fluid Antigen (CFA), Scolex Antigen (SA) and Membrane-Body Antigen (MBA)) and one metabolic antigen (Excretory-Secretory Antigen (ESA)) prepared from Cysticercus cellulosae originated from a naturally infected pig. Immunodominat proteins were determined using hyper immune rabbit sera. The Sodium Dodecyl Sulphate- Polyacrylamide Gel Electrophoresis (SDS-PAGE) profile of the antigens revealed different numbers and patterns of protein bands in the range of 11.80 to 176.74 kDa. The electrophoretic pattern of ESA also revealed a number of closely placed indistinct bands in the range of 20-34 kDa.. The proteins in the lower molecular weight range (< 30 kDa) in the case of WCA, CFA, ESA, and the medium molecular weight range (30-60 kDa) in the case of SA and MBA were observed to be immunodominant in the Enzyme-Linked Immunoelectrotransfer Blot (EITB) assay. These immunodominant proteins could be further investigated as potential diagnostic antigens in the serodiagnosis of cysticercosis in pigs, and neurocysticercosis in human patients.
Keywords
cysticercosis; pig; antigens; EITB; immunodominant proteins
Hrčak ID:
283891
URI
Publication date:
26.9.2022.
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