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Veterinary Archives, Vol. 92 No. 4, 2022.

Short communication, Note

https://doi.org/10.24099/vet. arhiv.1346

Detection of virulence genes and determination of the antimicrobial susceptibility of Escherichia coli isolates with mastitis in Mashhad, Iran – a short communication

Fateme Aflakian ; Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Mehrnaz Rad ; Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Himen Salimizand ; Vaccine and Infectious Disease Organization Saskatoon, SK, Canada; Department of Vaccinology and Immunotherapeutics, School of Public Health, University of Saskatchewan, Saskatoon, SK, Canada
Ali Nemati ; Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Abolfazl Rafati Zomorodi ; Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Science, Shiraz, Iran; Student Research Committee, Shiraz University of Medical Science, Shiraz, Iran

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Abstract

The purpose of this study was to determine the virulence genes and antimicrobial resistance patterns of Escherichia coli isolated from milk samples of cows with bovine mastitis. Forty-seven E. coli isolates from clinical mastitis milk samples, from five dairy farms in Northeast of Iran, were subjected to multiplex PCR to determine virulence genes stx1, stx2, eaeA, hlyA, sta, F4, F17, fliC, and rfbE. In addition, antimicrobial susceptibility was assessed by applying disk diffusion methods. The eaeA and stx1 genes were most frequently detected in 42 (89.3%) and 34 (72.3%) isolates, respectively. However, the least frequent gene was F41 as it was found in only one isolate (2.1%). Furthermore, 9 out of 47 isolates were hlyA positive, and four isolates harbored the sta gene. The antimicrobial susceptibility demonstrated the highest resistance against lincomycin (100%) and neomycin (91.4%). Since these bacteria represent a high-risk pathogen on farms, the emergence of multiple antibiotic-resistant and pathogenic E. coli strains should be of great concern for public health.

Keywords

E. coli, virulence genes; antimicrobial resistance; mastitis

Hrčak ID:

283900

URI

https://hrcak.srce.hr/283900

Publication date:

26.9.2022.

Article data in other languages: croatian

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