Original scientific paper

https://doi.org/10.2478/aiht-2022-73-3650

Redoks-status i koncentracije biometala u Wistar štakora nakon subakutnog izlaganja fluoridu i zaštitni učinak selena

Jelena Radovanović orcid.org/0000-0002-3005-7943 ; University of Belgrade School of Dental Medicine, Clinic for Paediatric and Preventive Dentistry, Belgrade, Serbia 2 University of Belgrade, Vinča Institute of Nuclear Sciences, Department of Radiobiology and Molecular Genetics, Belgrade, Serbia
Biljana Antonijević ; University of Belgrade Faculty of Pharmacy, Department of Toxicology “Akademik Danilo Soldatović”, Belgrade, Serbia
Katarina Baralić ; University of Belgrade Faculty of Pharmacy, Department of Toxicology “Akademik Danilo Soldatović”, Belgrade, Serbia
Marijana Ćurčić ; University of Belgrade Faculty of Pharmacy, Department of Toxicology “Akademik Danilo Soldatović”, Belgrade, Serbia
Danijela Đukić-Ćosić ; University of Belgrade Faculty of Pharmacy, Department of Toxicology “Akademik Danilo Soldatović”, Belgrade, Serbia
Zorica Bulat ; University of Belgrade Faculty of Pharmacy, Department of Toxicology “Akademik Danilo Soldatović”, Belgrade, Serbia
Dragana Javorac orcid.org/0000-0003-2744-2565 ; University of Belgrade Faculty of Pharmacy, Department of Toxicology “Akademik Danilo Soldatović”, Belgrade, Serbia
Aleksandra Buha Đorđević ; University of Belgrade Faculty of Pharmacy, Department of Toxicology “Akademik Danilo Soldatović”, Belgrade, Serbia
Jelena Kotur-Stevuljević orcid.org/0000-0002-6980-3069 ; University of Belgrade Faculty of Pharmacy, Department of Biochemistry, Belgrade, Serbia
Emina Sudar-Milovanović orcid.org/0000-0002-2273-5310 ; University of Belgrade, Vinča Institute of Nuclear Sciences, Department of Radiobiology and Molecular Genetics, Belgrade, Serbia
Evica Antonijević Miljaković ; University of Belgrade Faculty of Pharmacy, Department of Toxicology “Akademik Danilo Soldatović”, Belgrade, Serbia
Miloš Beloica ; University of Belgrade School of Dental Medicine, Clinic for Paediatric and Preventive Dentistry, Belgrade, Serbia
Zoran Zoran orcid.org/0000-0003-2622-6043 ; University of Belgrade School of Dental Medicine, Clinic for Paediatric and Preventive Dentistry, Belgrade, Serbia

Abstract

Cilj ovog istraživanja bio je utvrditi djelovanje 150 mg/L natrijevog fluorida (NaF) na redoks-status i koncentracije esencijalnih elemenata [bakar (Cu), željezo (Fe) i cink (Zn)] u krvi, jetri, bubrezima, mozgu i slezeni Wistar štakora te mogući zaštitni učinak selena (Se) od toksičnosti prouzročene fluoridom (F-). Mužjaci Wistar štakora nasumično su razvrstani u četiri skupine (n=5), nakon čega su 28 dana konzumirali običnu vodu ili vodu s otopinom NaF 150 mg/L, NaF 150 mg/L + Se 1,5 mg/L ili Se 1,5 mg/L. Izloženost fluoridu dovela je do poremećaja redoks-parametara i koncentracija istraživanih biometala. Utvrđene su povišene razine superoksid aniona (O2.-) i malondialdehida (MDA) u krvi i mozgu, smanjena aktivnost superoksid dismutaze (SOD1) u jetri te njezin porast u mozgu i bubrezima. Nadomjesni Se u kombinaciji s NaF pozitivno je utjecao na razine MDA, SOD1 i O2.- u krvi, mozgu i bubrezima, a sâm Se smanjio je razine SH skupina u jetri i bubrezima. Izloženost fluoridu uzrokovala je sniženje, ali i porast koncentracija biometala. Nužna su dodatna istraživanja kako bi se ispitali antioksidacijski učinci Se na toksičnost izazvanu F-.

Keywords

Cu; Fe; MDA; NaF; natrijev fluorid; O2 .-; oksidacijski stres; Se; SOD1; subakutna toksičnost; Zn

Hrčak ID:

283982

URI

https://hrcak.srce.hr/283982

Publication date:

29.9.2022.

Article data in other languages: english

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