Original scientific paper

Multiplex Mutagenically Separated Polymerase Chain Reaction Assay for Rapid Detection of Human Mitochondrial DNA Variations in Coding Area

Jin Wu ; Institute of Forensic Medicine, Sichuan University, China
Peng Ran ; Institute of Forensic Medicine, Sichuan University, China
Yingbi Li ; Institute of Forensic Medicine, Sichuan University, China
Jing Yan ; Institute of Forensic Medicine, Sichuan University, China
Miao Liao ; Institute of Forensic Medicine, Sichuan University, China
Yiping Hou ; Institute of Forensic Medicine, Sichuan University, China
Lin Zhang ; Institute of Forensic Medicine, Sichuan University, China

Abstract

Recently, it has been recognized that information in the mitochondrial
DNA (mtDNA) coding region can provide additional forensic discrimination
with respect to the standard typing of the D-loop region, increasing
the forensic power of mtDNA testing, which is sometimes rather
limited. In the present study, we simultaneously typed ten single nucleotide
polymorphisms (SNP) in the coding region by use of mutagenically
separated polymerase chain reaction (MS-PCR) in the Chinese Chengdu
population. This technique, in which different-size allele-specific primers
were used, specifically amplified both alleles of mtDNA in the same
reaction. Subsequent gel electrophoresis showed ten of the allelic products
of different loci. Using multiplex MS-PCR, 30 primers were added
simultaneously into one reaction tube to identify ten SNPs. The mtDNA
variations of 160 individuals from the Chinese Chengdu population
were examined and classified into 18 haplotypes. The multiplex MS-PCR
method is suitable for large-scale screening studies of mtDNA variability
because it is both rapid and economical.

Keywords

Mitochondrial DNA; Single nucleotide polymorphisms; mutagenically separated PCR; Chinese chengdu population

Hrčak ID:

26087

URI

https://hrcak.srce.hr/26087

Publication date:

15.2.2008.

Article data in other languages: croatian

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