Original scientific paper

Y-chromosomal Short Tandem Repeat Haplotypes in Southern Croatian Male Population Defined by 17 Loci

Jelena Ljubković ; Department of Pethology and Forensic Medicine, Split University Hospital and School of Medicine, Split, Croatia
Angela Stipišić ; Public Health Institute of Split-Dalmatia Country, Split, Croatia
Davorka Sutlović orcid.org/0000-0002-0102-7251 ; Department of Pethology and Forensic Medicine, Split University Hospital and School of Medicine, Split, Croatia
Marija Definis-Gojanović ; Department of Pethology and Forensic Medicine, Split University Hospital and School of Medicine, Split, Croatia
Kajo Bučan ; Department of Ophtalmology, Split University Hospital and School of Medicine, Split, Croatia
Šimun Anđelinović ; Department of Pethology and Forensic Medicine, Split University Hospital and School of Medicine, Split, Croatia

Abstract

Aim To define the Y-chromosome genetic structure in a sample of
men from southern Croatia.
Methods Blood samples were collected from 166 unrelated healthy
men from southern Croatia at the Department of Forensic Medicine
and Biochemical Laboratory of University Hospital Split between
2004 and 2007. Genomic DNA was extracted using the standard
procedures. Seventeen Y-chromosome short tandem repeat (YSTR)
polymorphic loci (DYS456, DYS389I, DYS390, DYS389II,
DYS458, DYS19, DYS385, DYS393, DYS391, DYS439, DYS635,
DYS392, GATAH4, DYS437, DYS438, and DYS448) were analyzed
using AmpFlSTR Yfiler Polymerase Chain Reaction Amplification
Kit.
Results We observed 152 different haplotypes. Total haplotype
diversity was 0.997289 and 141 haplotypes (84.49%) were unique.
The most common haplotype was shared by only 4 men in the study
sample. The locus diversity ranged between 0.21292 for DYS392
and 0.75546 for DYS439 locus.
Conclusion The Y-chromosome structure in men from southern
Croatia is very diverse. Combination of Y chromosome 17 STR loci
may be used as a powerful tool for individual identification and parentage
analysis in the southern Croatian male population.

Keywords

forensic science; Haplotype; Y-chromosomal STR; population data; Southern Croatia

Hrčak ID:

26153

URI

https://hrcak.srce.hr/26153

Publication date:

15.4.2008.

Article data in other languages: croatian

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