Skip to the main content

Journal of Electrochemical Science and Engineering, Vol. 12 No. 6, 2022.

Original scientific paper

https://doi.org/10.5599/jese.1455

Screen-printed carbon electrode/natural silica-ceria nanocomposite for electrochemical aptasensor application

Salma Nur Zakiyyah orcid id orcid.org/0000-0003-0985-5980 ; Department of Chemistry, Faculty of Mathematics and Sciences, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang km. 21 Jatinangor, Sumedang 45363, Indonesia
Diana Rakhmawaty Eddy ; Department of Chemistry, Faculty of Mathematics and Sciences, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang km. 21 Jatinangor, Sumedang 45363, Indonesia
M. Lutfi Firdaus ; Graduate School of Science Education, bengkulu University, Jl. W.R. Supratman Kandang Limun, Bengkulu 38371, Indonesia
Toto Subroto ; Bionformatics and Biomolecular Research Center, Universitas Padjadjaran, Jl. Singaperbangsa 2, Bandung 40132, Indonesia
Yeni Wahyuni Hartati orcid id orcid.org/0000-0003-1463-6352 ; Department of Chemistry, Faculty of Mathematics and Sciences, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang km. 21 Jatinangor, Sumedang 45363, Indonesia

Full text: english pdf 1.009 Kb

page 1225-1242

downloads: 222

cite

Abstract

A nanocomposite of natural silica and ceria was synthesized to modify a screen-printed carbon electrode (SPCE) to develop an aptasensor to detect epithelial sodium channel (ENaC) protein in urine as a biomarker of hypertension. The method steps were the synthesis of natural silica-ceria nanocomposite using the hydrothermal method, obtaining of natural silica nanoparticles from the extraction of alkaline silica sand and ceria nanoparticles from cerium nitrate, modification of SPCE/natural silica-ceria, immobilization of aptamer through streptavidin-biotin, and detection of ENaC protein conc­entration. Box-Behnken’s design was employed to determine the optimal con­ditions of aptamer concentration (0.5 μg mL-1), streptavidin incubation time (30 min), and aptamer incubation time (1 hour), respectively. Differential pulse voltam­metry (DPV) characterization of the developed electrochemical aptasensor revealed that the [Fe(CN)6]3-/4- redox peak current increased from 3.190 to 9.073 μA, with detection and quantification limits of 0.113 and 0.343 ng mL-1, respectively. The method is proven as a simple and rapid method to monitor ENaC levels in urine samples.

Keywords

Silica - ceria; synthesis composite; aptamer; ENaC protein; hypertension biomarker; voltammetry

Hrčak ID:

287001

URI

https://hrcak.srce.hr/287001

Publication date:

7.12.2022.

Visits: 648 *