Original scientific paper

CELL DEATH DIFFERENTIATION IN BLACK HEADED RAMS SPERMATOZOA, USING FLUORESCENT LABELED ANNEXIN V

M. Ivanova-Kicheva ; Institute of Biology and Immunology of Reproduction “Acad. Kiril Bratanov” – Bulgarian Academy of Sciences, Sofi a Bulgaria
A. Kukov ; Institute of Biology and Immunology of Reproduction “Acad. Kiril Bratanov” – Bulgarian Academy of Sciences, Sofi a Bulgaria
D. Daskalova ; Institute of Biology and Immunology of Reproduction “Acad. Kiril Bratanov” – Bulgarian Academy of Sciences, Sofi a Bulgaria
E. Solenkova ; Institute of Biology and Immunology of Reproduction “Acad. Kiril Bratanov” – Bulgarian Academy of Sciences, Sofi a Bulgaria
I. Nikolov ; Institute of Biology and Immunology of Reproduction “Acad. Kiril Bratanov” – Bulgarian Academy of Sciences, Sofi a Bulgaria
L. Todorov ; Institute of Biology and Immunology of Reproduction “Acad. Kiril Bratanov” – Bulgarian Academy of Sciences, Sofi a Bulgaria

Abstract

Double staining kit of Annexin V Cy3.18/6-CFDA was used to investigate the changes in phospholipide asymmetry after treating sperm cells with dexamethasone. The % of spermatozoa with registered translocation of PS in treated with dexamethazone groups at the 10-th min and in control no treated varied from 2.74%±0.65 to 2.30%±0.89. After the 5 hour of incubation these % increased to 39.83±3.33 for the treated group and 23.44±1.12 for the control. It was concluded that Annexin V binding assay is more sensitive in the detection of deterioration in membrane function than other conventional methods such as motility analysis and supravital techniques.

Keywords

spermatozoa; plasma membrane; phosphatidylserine

Hrčak ID:

26323

URI

https://hrcak.srce.hr/26323

Publication date:

28.7.2008.

Article data in other languages: bulgarian

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