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Acta Pharmaceutica, Vol. 58 No. 4, 2008.

Original scientific paper

https://doi.org/10.2478/v10007-008-0022-3

Protective role of the calcium channel blocker amlodipine against mitochondrial injury in ishemia and reperfusion injury of rat liver

CHATTOPADHYAY PRONOBESH ; Cellular and Microbiology Laboratory, College of Pharmacy, IFTM, Moradabad-244001, India; Birla Institute of Technology and Sciences, Pilani, India
AHER VAIBHAV ; Cellular and Microbiology Laboratory, College of Pharmacy, IFTM, Moradabad-244001, India
CHAUDHURY PALLAB ; National Biotechnology Center, Indian Veterinary Research Institute, Izatnagar, India
WAHI ARUN KUMAR ; Cellular and Microbiology Laboratory, College of Pharmacy, IFTM, Moradabad-244001, India

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Abstract

Ca2+ accumulation and Ca2+ overloading into mitochondria are responsible for the cell abnormality associated with ischemia and reperfusion injury. The present study was aimed to evaluate the efficacy of Ca2+ channel blocker- amlodipine on the mitochondrial Ca2+ accumulation, mitochondrial antioxidant status and mitochondrial respiratory enzymes in ischemic - reperfusion (I/R) induced liver injury. I/R injury induced mitochondrial damage in rats was assessed in terms of decrease in activities (p< 0.05) of respiratory marker enzymes (malate dehydrogenase, succinate dehydrogenase and NADH –dehydrogenase), mitochondrial antioxidant enzymes (glutathione, superoxide dismutase, catalase), level of lipid peroxidation.(LPO), and Ca2+ accumulation were assessed as marker of mitochondrial damage.
Mitochondrial damage was confirmed by transmission electron microscopic (TEM) examination. Pretreatment with amlodipine effectively counteracted the alternation in mitochondrial enzymes induced by ischemia-reperfusion liver damage. TEM study confirms the restoration of cellular normalcy and accredits the cytoprotective role of amlodipine against I/R induced hepatic injury. On the basis of our findings it may be concluded that amlodipine not only possesses Ca2+ channel antagonist properties but it may also reduce the extent of mitochondrial damage by its antioxidant activity.

Keywords

calcium channel blocker; amlodipine; ischemic and reperfusion injury; mitochondrial injury

Hrčak ID:

26481

URI

https://hrcak.srce.hr/26481

Publication date:

1.12.2008.

Article data in other languages: croatian

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