Veterinary Archives, Vol. 78 No. 4, 2008.
Original scientific paper
Diagnosis of Echinococcus granulosus infection in dogs by a coproantigen sandwich ELISA
Prathapan Rema Prathiush
; Centre of Advanced Studies, Department of Veterinary Parasitology, Veterinary College, Karnataka Veterinary, Animal and Fisheries Sciences University, Bangalore, India
Placid Eugene D’Souza
; Centre of Advanced Studies, Department of Veterinary Parasitology, Veterinary College, Karnataka Veterinary, Animal and Fisheries Sciences University, Bangalore, India
Ananda K. Javare Gowda
; Centre of Advanced Studies, Department of Veterinary Parasitology, Veterinary College, Karnataka Veterinary, Animal and Fisheries Sciences University, Bangalore, India
Abstract
Echinococcosis is a near-cosmopolitan zoonosis caused by the adult or larval stages of tapeworms belonging to the genus Echinococcus. Cystic echinococcosis in food animals is highly prevalent in India in general and in the Karnataka state in particular. A sandwich ELISA was standardized and evaluated in field conditions for coproantigen detection of Echinococcus granulosus infection in dogs. Field fecal supernatants were titrated against positive dog serum. A total of 368 fecal supernatants were tested and 16 samples were found to be positive in sandwich ELISA. Sensitivity and specificity was calculated by taking necropsy as the standard. The sensitivity was found to be 100 percent and the specificity was 96.94 percent.
Keywords
Echinococcus; coproantigen; dog; fecal supernatant; sandwich ELISA
Hrčak ID:
26537
URI
Publication date:
20.8.2008.
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