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Chemical and Biochemical Engineering Quarterly, Vol. 22 No. 3, 2008.

Original scientific paper

Kinetically Controlled Synthesis of Cefaclor with Immobilized Penicillin Acylase in the Presence of Organic Cosolvents

Ye-Wang Zhang ; School of Pharmaceutics, Jiangsu University, Zhenjiang, 212013, P. R. China; State Key Laboratory of Bioreator Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, P. R. China
Shu-Lai Liu ; State Key Laboratory of Bioreator Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, P. R. China
Dong-Zhi Wei ; State Key Laboratory of Bioreator Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, P. R. China
Rui-Jiang Liu ; School of Pharmaceutics, Jiangsu University, Zhenjiang, 212013, P. R. China; United research center of Jiangsu University and Sopo Xingda Pharmaceutical Co. Ltd., Zhenjiang, 212013, Zhenjiang, P. R. China
Xi-Ming Xu ; School of Pharmaceutics, Jiangsu University, Zhenjiang, 212013, P. R. China

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Abstract

Enzymatic syntheses of cefaclor with immobilized penicillin acylase in organic cosolvents under kinetic control were carried out. KcPGA from Kluyvera citrophila was selected as the best catalyst among the three species of immobilized penicillin acylase. Ethylene glycol, glycerol, methanol, ethyl estate and polyethyleneglycol (PEG) were selected accordingly and cefaclor syntheses were preformed respectively. Best results in terms of yield were obtained in ethylene glycol, with which further studies were investigated and the maximum yield was Y = 93.5 %. The optimal conditions were pH 6.5, temperature θ = 5 °C, 3 mol D-phenylglycine methyl ester (PGME) per mol 7-aminodesacetoxymehtyl-3-chlorocephalosporin acid (7ACCA) and x = 30 % ethylene glycol fraction. Under above mentioned conditions, the yield was Y = 91.1 %.

Keywords

3-chloro-7-D-(2-phenylglycinamide)-3-cephem-4-carboxylic acid; organic cosolvents; Penicillin acylase; enzymatic synthesis

Hrčak ID:

26767

URI

https://hrcak.srce.hr/26767

Publication date:

25.9.2008.

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