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Original scientific paper

Analysis of Fibroblast Growth Factor Influence on Growth and Developmental Potential of Rat Foetuses in the In Vitro Culture Model

Svjetlana Marić
Floriana Bulić-Jakuš
Tatjana Belovari
Stjepan Krčmar

Full text: english pdf 98 Kb

page 911-918

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The fibroblast growth factor’s (FGF) influence on the growth and differentiation of 8- and 9- day-old rat foetus has
been studied, whereas foetuses were grown in an in vitro culture model. Proliferation was analysed by the expression of
proliferating cell nuclear antigen (PCNA). It was established that the usage of FGF in the first period of the culture lowers
the growth no matter the foetus age at the moment of culturing and no matter whether it is a medium with or without
a serum. If FGF is applied in the second culture period, it also lowers the growth, however younger foetuses in the in vitro
culture model are more sensible to FGF negative influence.When FGF was applied in a lower concentration the growth of
whole foetuses was improved in the in vitro culture model, which shows that the FGF influence on growth depends on the
concentration. Stereological analyses have been done and showed that, in the in vitro culture model, FGF has no influence
on proliferating cartilage tissue, but it stimulates the survival of nervel tissue cells. It has been shown that the quantitative
research of growth processes in cultivated foetuses can precisely be done by combining classic methods of measuring
whole foetus diameters and analysing the expression of proliferating antigen.


FGF; embryo; rat; development

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