Original scientific paper
https://doi.org/10.2478/aiht-2023-74-3692
Different damaging effects of volatile anaesthetics alone or in combination with 1 and 2 Gy gamma-irradiation in vivo on mouse liver DNA: a preliminary study
Vesna Benković
orcid.org/0000-0003-3307-1684
; University of Zagreb Faculty of Science, Department of Biology, Zagreb, Croatia
Mirta Milić
orcid.org/0000-0002-9837-7185
; Institute for Medical Research and Occupational Health, Mutagenesis Unit, Zagreb, Croatia
Nada Oršolić
orcid.org/0000-0001-5102-3606
; University of Zagreb Faculty of Science, Department of Biology, Zagreb, Croatia
Anica Horvat Knežević
; University of Zagreb Faculty of Science, Department of Biology, Zagreb, Croatia
Gordana Brozović
; Sestre Milosrdnice University Hospital, Clinic for Tumours, Department of Anaesthesiology, Reanimatology, and Intensive Care, Zagreb, Croatia 4 University of Osijek Faculty of Dental Medicine and Health, Osijek, Croatia
Nikola Borojević
; National Health Service Foundation Trust, Warrington and Halton Teaching Hospitals, Warrington, United Kingdom
Abstract
As the number of radiotherapy and radiology diagnostic procedures increases from year to year, so does the use of general volatile anaesthesia (VA). Although considered safe, VA exposure can cause different adverse effects and, in combination with ionising radiation (IR), can also cause synergistic effects. However, little is known about DNA damage incurred by this combination at doses applied in a single radiotherapy treatment. To learn more about it, we assessed DNA damage and repair response in the liver tissue of Swiss albino male mice following exposure to isoflurane (I), sevoflurane (S), or halothane (H) alone or in combination with 1 or 2 Gy irradiation using the comet assay. Samples were taken immediately (0 h) and 2, 6, and 24 h after exposure. Compared to control, the highest DNA damage was found in mice receiving halothane alone or in combination with 1 or 2 Gy IR treatments. Sevoflurane and isoflurane displayed protective effects against 1 Gy IR, while with 2 Gy IR the first adverse effects appeared at 24 h post-exposure. Although VA effects depend on liver metabolism, the detection of unrepaired DNA damage 24 h after combined exposure with 2 Gy IR indicates that we need to look further into the combined effects of VA and IR on genome stability and include a longer time frame than 24 h for single exposure as well as repeated exposure as a more realistic scenario in radiotherapy treatment.
Keywords
alkaline comet assay; halothane; ionising irradiation; isoflurane; sevoflurane
Hrčak ID:
296529
URI
Publication date:
28.3.2023.
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