Original scientific paper
Association of Angiotensin-Converting Enzyme Insertion-Deletion Polymorphism with Preeclampsia
Berivoj Mišković
Jadranka Sertić
Ana Stavljenić-Rukavina
Feodora Stipoljev
Abstract
The aim of this study was to determine if insertion-deletion polymorphism of angiotensin-converting enzyme is a risk
factor for the development of preeclampsia. Sixty women with preeclampsia and 50 normotensive pregnant women were
included in this study. Preeclampsia was defined as blood pressure > 140/90 mmHg in a previously normotensive women
with proteinuria >300 mg/L in a 24-hours. Twelve women also had preeclampsia in previous pregnancy. The genotyping
of polymorphism in the intron 16 of the angiotensin-converting enzyme was performed by the polymerase chain reaction
followed by the agarose electrophoresis. The patients were divided into three groups according to the presence (I)
or absence (D) of insertional polymorphism (II, ID, and DD). Genotype distribution and allele frequencies were compared
by Mantel-Haenszel c
2 testing. The frequency of DD genotype was not significantly higher in women with preeclampsia
(26/60)than in the control group (14/50, p=0.096). The D allele frequency was significantly higher in 17 women with
preeclampsias who required delivery before 34 weeks of pregnancy (0.735), than in 43 women in whom obstetric complications
took place after 34 weeks of pregnancy (0.56, p=0.036). The D allele frequency was 0.83 in women having recurrent
preeclampsia, i.e. significantly higher compared with women, who were for the first time, experienced preeclampsia
(0.57, p=0.013). This study showed a significantly positive association between D allele frequency and risk of recurrent
preeclampsia and preterm delivery before 34 weeks of pregnancy. The deletion genotype could be an important contributing
factor for an early onset and recurrent preeclampsia.
Keywords
preeclampsia; Caucasians; renin-angiotensin system; gene; polymorphism
Hrčak ID:
27202
URI
Publication date:
7.7.2008.
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