Original scientific paper
The Antioxidant Power and Level of Lipid Peroxidation Products in the Sera of Apparently Healthy Adult Males
Višnja Katalinić
Ilza Salamunić
Senijo Pažanin
Rosanda Mulić
Marija Milišić
Darko Ropac
Abstract
The aim of this study was to determine the antioxidant potential of the serum and the level of lipid oxidation products
in the sera of apparently healthy adult males. The »antioxidant power« of the serum, defined as the ability to reduce ferric
ions by antioxidants from the serum (FRAP), was taken as the indicator of total antioxidation potential. The formation
of lipid oxidation products was evaluated as thiobarbituric reactive species serum test (TBARS). The ferrous oxidation
in xylenol orange version 2 (FOX2) assay coupled with triphenylphosphine was used for measurement of total sera
hydroperoxides (ROOHs). The following biochemical variables were determined in the sera: aspartat aminotranspherase
(AST), alanine aminotranspherase (ALT), g-glutamyl transpherase (GGT), bilirubin, glucose, creatinine, cholesterol,
triglycerides and hemoglobin. Blood sera from apparently healthy subjects (166 adult males) were analyzed. Median
age of study participants was 36 years (range 25–50 years). The X±SD sera FRAP level of the 166 apparently
healthy adult males was 1047±131 mmol/L (779–1410 range). The X±SD level of sera TBARS was 1.2±0.3 mmol/L of the
sera (0.5–2.2 range). Compared with the level of TBARS in the sera, the level of FOX2-ROOH was significantly higher.
The X±SD level of lipid hydroperoxides in the fresh sera, determined as FOX2, was 3.9±1.5 mmol/L of the sera (1.9–6.9
range). Observation of correlation between FRAP and determined biochemical variables in the sera have confirmed a statistically
significant linear correlation between sera FRAP and bilirubin, hemoglobine, glucose, ALT and triglycerides
(p<0.05). The results of sera FRAP, TBARS and FOX2 levels can help in estimating the antioxidant status of humans.
Significant correlation between the antioxidant power of blood serum and particular biochemical parameters indicates
the complexity of defence mechanisms and various molecules involved in increasing the reduction power of the serum.
Keywords
blood sera; antioxidant power; lipid peroxides; FRAP; FOX2; TBARS
Hrčak ID:
27300
URI
Publication date:
4.1.2007.
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