Original scientific paper

Association between Pro12Ala and His477His polymorphisms in PPARG gene and insulin resistance in patients with polycystic ovary syndrome

Jana Dragojevič ; Department of Clinical Biochemistry, Faculty of Pharmacy, University of Ljubljana, Ljubljana, Slovenia
Janja Marc orcid.org/0000-0001-6013-9758 ; Department of Clinical Biochemistry, Faculty of Pharmacy, University of Ljubljana, Ljubljana, Slovenia
Barbara Mlinar ; Department of Clinical Biochemistry, Faculty of Pharmacy, University of Ljubljana, Ljubljana, Slovenia

Abstract

Background: Insulin resistance is characterized by attenuated response of tissues to insulin action and is associated with obesity, type 2 diabetes mellitus, metabolic syndrome, lipodystrophies, polycystic ovary syndrome (PCOS) and chronic infection. Insulin resistance is present in 50%—70% of PCOS patients. Peroxisome proliferator-activated receptor γ (PPARγ) is a nuclear receptor which controls transcription of genes involved in free fatty acid uptake and lipogenesis, and is essential for diferentiation and survival of adipo-cytes. The aim of our pilot study was to investigate the association between Pro12Ala and His477His polymorphisms in PPARG gene with insulin resistance in PCOS patients.
Patients and methods: The study included 69 PCOS patients. Genotype frequencies were analyzed by PCR-RFLP methods. Fasting glucose and glucose after oral glucose tolerance test, insulin, and high sensitivity C-reactive protein (hsCRP), body mass, height, waist circumference, systolic and diastolic blood pressure, body mass index (BMI) and homeostasis model assessment (HOMA) index were measured by routine methods or calculated from the findings obtained.
Results: A signi;cant association was found between the Ala allele of the Pro12Ala polymorphism and lower BMI (P = 0.040) and between the T allele of the His477His polymorphism and lower hsCRP (P = 0.047). The Pro12Ala and the His477His polymorphisms were in considerable linkage disequilibrium (D' = 0.727). Diplotype analysis showed no association.
Conclusions: In our preliminary study, we found a significant association between the Ala allele of the Pro12Ala polymorphism and lower BMI, and between the T allele of the His477His polymorphism and lower hsCRP. However, none of the two polymorphisms, individually or in combination (diplotype), was associated with fasting plasma insulin and glucose concentrations and HOMA index that are characteristic of insulin resistance. We therefore conclude that the polymorphisms investigated are not associated with insulin resistance in PCOS patients.

Keywords

PPARγ; polymorphism; insulin resistance; PCOS; hsCRP

Hrčak ID:

27338

URI

https://hrcak.srce.hr/27338

Publication date:

6.10.2008.

Article data in other languages: croatian

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