Original scientific paper
https://doi.org/10.15644/asc57/3/4
The Antibacterial and Antifungal Activity of Chlorhexidine Diacetate Incorporated into Acrylic Resins Used in Provisional Restorations
Secil Ozkan Ata
; Department of Prosthodontics, Faculty of Dentistry, Osmangazi University, Eskisehir, Turkey
Canan Akay
; Department of Prosthodontics, Faculty of Dentistry, Osmangazi University, Eskisehir, Turkey; Advanced Material Technologies Application and Research Center, Osmangazi University, Eskisehir, Turkey;; Translational Medicine Research and Clinical Center, Osmangazi University, Eskisehir, Turkey
Emre Mumcu
; Department of Prosthodontics, Faculty of Dentistry, Osmangazi University, Eskisehir, Turkey; Advanced Material Technologies Application and Research Center, Osmangazi University, Eskisehir, Turkey;; Translational Medicine Research and Clinical Center, Osmangazi University, Eskisehir, Turkey
Demet Erdonmez
; Duzce University, Faculty of Pharmacy, Department of Pharmaceutical Microbiology, Duzce, Turkey
Abstract
Objective: The surface of provisional restorations applied before conventional or implant- supported fixed restorations may cause bacterial or fungal biofilm formation. The aim of this study was to evaluate the antimicrobial activity of acrylic resins used in provisional restorations modified with chlorhexidine diacetate. Methods: 120 cylindrical, auto-polymerized resin samples modified with chlorhexidine diacetate were prepared at concentrations of 0 (control), 1, 3, 5 wt %. The antimicrobial activity was examined against Streptococcus mutans, Enterococcus faecalis, and Candida albicans using Crystal Violet quantification, MTT assay, and Scanning Electron Microscopy. Data were analyzed by ANOVA and paired sample t-tests (α=0.05). Results: The addition of chlorhexidine diacetate influenced the growth rate and metabolic activity of microorganisms. The antimicrobial effect against C. albicans and S. mutans statistically increased with the percentage of chlorhexidine diacetate. E. faecalis bacteria were less affected by chlorhexidine diacetate compared to other pathogens. Conclusion: It has been shown that the effectiveness of CHDA in inhibiting the proliferation of microorganisms correlated positively with increasing concentration levels. More research is needed to confirm the impact of different chlorhexidine concentrations on the mechanical properties, clinical efficacy, and antimicrobial properties of CDHA.
Keywords
Anti-Infective Agents; Chlorhexidine; Acrylic Resins; Temporary Dental Restoration; Provisional Restorations; Prosthodontics; Antimicrobial Effect
Hrčak ID:
308190
URI
Publication date:
27.9.2023.
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