Original scientific paper
https://doi.org/10.2478/aiht-2023-74-3768
L-glutamic acid-g-poly hydroxyethyl methacrylate nanoparticles: acute and sub-acute toxicity and biodistribution potential in mice
Buket Bakan
Fatih Oltulu
; Ege University Faculty of Medicine, Department of Histology and Embryology, Izmir, Turkey
Yeliz Yıldırım
; Ege University Faculty of Science, Department of Chemistry, Izmir, Turkey
Sinan Akgöl
; Ege University Faculty of Science, Department of Biochemistry, Izmir, Turkey
Nefise Ülkü Karabay Yavaşoğlu
; Ege University Faculty of Science, Department of Biology, Izmir, Turkey
Abstract
The aim of this safety study in mice was to determine in vivo toxicity and biodistribution potential of a single and multiple doses of L-glutamic acid-g-p(HEMA) polymeric nanoparticles as a drug delivery system. The single dose did not cause any lethal effect, and its acute oral LD50 was >2.000 mg/kg body weight (bw). Multiple doses (25, 50, or 100 mg/kg bw) given over 28 days resulted in no significant differences in body and relative organ weights compared to control. These results are supported by biochemical and histological findings. Moreover, nanoparticle exposure did not result in statistically significant differences in micronucleus counts in bone marrow cells compared to control. Nanoparticle distribution was time-dependent, and they reached the organs and even bone marrow by hour 6, as established by ex vivo imaging with the IVIS® spectrum imaging system. In conclusion, L-glutamic acid-g-p(HEMA) polymeric nanoparticles appear biocompatible and have a potential use as a drug delivery system.
Keywords
biocompatibility; blood biochemistry; genotoxicity; histology; in vivo toxicity; micronucleus test; polymers
Hrčak ID:
308238
URI
Publication date:
27.9.2023.
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