Original scientific paper
https://doi.org/10.15255/CABEQ.2023.2182
Amoxicillin Biodegradation with Bacillus subtilis and Pseudomonas aeruginosa: Characterization of Relevant Degradation Products
S. Kovačić
; Pliva Croatia Ltd., Prilaz baruna Filipovića 25, 10000 Zagreb, Croatia
V. Lovrinčević
; University of Zagreb, Faculty of Chemical Engineering and Technology, Department of Organic Chemistry, Marulićev trg 19, 10000 Zagreb, Croatia
M. Vuković Domanovac
; University of Zagreb, Faculty of Chemical Engineering and Technology, Department of Industrial Ecology, Marulićev trg 19, 10000 Zagreb, Croatia
D. Vuk
; University of Zagreb, Faculty of Chemical Engineering and Technology, Department of Organic Chemistry, Marulićev trg 19, 10000 Zagreb, Croatia
Abstract
The widespread use of antibiotics can result in the release of a large number of residues into the environment. In order to reduce the exposure risk, considerable research has been carried out in the field of antibiotic residues. We aim to explore the possibilities of antibiotic amoxicillin biodegradation in laboratory scale by adding microbiological cultures, Pseudomonas aeruginosa (Gram-negative) and Bacillus subtilis (Gram-positive), which are a type of bacteria that are commonly found in the environment, such as in soil and water. Apart from the degradation itself, special attention was paid to the identification of antibiotic residues. The biodegradation process was investigated, revealing a 99.2 % degradation efficiency at amoxicillin concentration of 0.2 mg mL–1, and four degradation products were identified. For reaction monitoring and identification of degradation products, UHPLC and LC-MS analyses were performed. Furthermore, to provide additional evidence, the products were independently synthesized and then compared spectrometrically with the obtained biodegradation products. These results could provide new aspects to the behavior of amoxicillin, and pave the way for further monitoring and studies of its residues in the environment.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Keywords
antibiotic residues; environment contamination; amoxicillin; biodegradation; biodegradation products
Hrčak ID:
308403
URI
Publication date:
4.10.2023.
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