Original scientific paper

https://doi.org/10.24099/vet.arhiv.2123

Razvoj kvantitativnog PCR testa temeljenog na SYBR Green I za identifikaciju cirkovirusa svinja 1, 2 i 3

Chang Liu ; Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, China
Yao Tian ; Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, China; College of Veterinary Medicine, Qingdao Agricultural University, China
Li-mei Zheng ; College of Veterinary Medicine, Qingdao Agricultural University, China
Ying-Ru Ma ; College of Veterinary Medicine, Qingdao Agricultural University, China
Jian-Li Shi ; Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, China
Chen Li ; Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, China; College of Veterinary Medicine, Qingdao Agricultural University, China
Shao-Jian Xu ; Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, China
Xiao-Yan Wu ; Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, China
Hong Han ; College of Veterinary Medicine, Qingdao Agricultural University, China
Jun Li ; Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, China *
Xian-Jie Han ; Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, China *

* Corresponding author.

Abstract

Među cirkovirusima svinja razlikujemo cirkovirus svinja tipa 1 (PCV1), cirkovirus svinja tipa 2 (PCV2) i cirkovirus svinja tipa 3 (PCV3). Posljednjih se godina pojavljuje koinfekcija ovim trima serotipovima, stoga je potrebno uspostaviti brzu, specifičnu i osjetljivu metodu kako bi se kvantitativnim PCR testom temeljenom na SYBR Green I mogli identificirati PCV1, PCV2 i PCV3. U ovom su istraživanju upotrijebljene specifične početnice te su optimizirani uvjeti rekacije za uspostavljanje kvantitativnog PCR-a u stvarnom vremenu. Rezultati su pokazali da su granice detekcije ovog testa 40,3 kopije/μL za PCV1, 25,2 kopije/μL za PCV2 i 22,4 kopije/μL za PCV3. Nije bilo križne reaktivnosti s virusom svinjske kuge (CSFV), virusom reproduktivnog i respiratornog sindroma svinja (PRRSV), virusom pseudobjesnoće svinja (PRV) i parvovirusom svinja (PPV). Koeficijenti varijacije unutar testa i među testovima bili su manji od 1 %. Rezultati analize 100 uzoraka sa sumnjom na PCV pokazali su da je stopa infekcije serotipom PCV1 bila 10% (10/100), PCV2 64% (64/100), a PCV3 52% (52/100). Stopa koinfekcije serotipovima PCV1 i PCV2 bila je 8% (8/100), PCV1 i PCV3 7% (7/100), a PCV2 i PCV3 26% (26/100). Koinfekcija svim trima serotipovima, PCV1, PCV2 i PCV3, bila je 7% (7/100). Metoda primjenjena u ovom istraživanju ima dobru specifičnost, osjetljivost i postojanost te je obećavajući alat za brzo otkrivanje serotipova PCV1, PCV2 i PCV3.

Keywords

cirkovirus svinja; specifičnost; osjetljivost; kvantitativni PCR test temeljen na SYBR Green I; metoda jednostruke identifikacije

Hrčak ID:

308824

URI

https://hrcak.srce.hr/308824

Publication date:

15.9.2023.

Article data in other languages: english

Visits: 567 *