Short communication, Note

https://doi.org/10.2478/v10007-008-0026-z

Effect of ampicillin and chloroquine on humoral immune response elicited by bovine albumin encapsulated in liposomes

JITENDER MADAN ; Department of Pharmaceutics, Hindu College of Pharmacy, Sonepat (Haryana), India
DINESH KAUSHIK ; Department of Pharmaceutics, Hindu College of Pharmacy, Sonepat (Haryana), India
SATISH SARDANA ; Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar (Haryana) India
DINA NATH MISHRA ; Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar (Haryana) India
SHALINDER KUMAR SINGH ; Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar (Haryana) India
BINOD KUMAR SINGH ; Department of Pharmacy, Kumaon University, Nainital, India

Abstract

Immune suppression resulting from chemoprophylaxis and potential drug interaction were investigated in experimental animals pre-medicated with ampicillin and chloroquine followed by immunization with bovine serum albumin bearing liposomes prepared by the reverse phase evaporation method. The prepared liposomes were evaluated for particle size, entrapment efficiency and in vitro release. Humoral immune response was measured in terms of systemic IgG antibody titre by the ELISA method. The present study showed that 7:3 molar ratio of soya phosphatidylcholine and cholesterol produced liposomes of mean diameter of 235.4  10.3 nm and entrapment efficiency of 41.3  3.2%. Ampicillin significantly (p < 0.05) decreased the antibody titre whereas chloroquine did not reduce the antibody titre significantly. The study will help in programming a new drug management and in characterization of vaccine-drug interaction.

Keywords

immunomodulation; liposomes; bovine albumin; ampicillin; chloroquine

Hrčak ID:

28797

URI

https://hrcak.srce.hr/28797

Publication date:

1.12.2008.

Article data in other languages: croatian

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