Original scientific paper

https://doi.org/10.24099/vet.arhiv.1968

Endometrial mRNA expression of key inflammatory genes and progesterone receptor in uterine tissue of bitches with endometritis

María Carla García Mitacek ; Animal Reproduction Research Institute (INIRA), Faculty of Veterinary Sciences, National University of La Plata, La Plata, Buenos Aires, Argentina; National Scientific and Technical Research Council (CONICET), Godoy Cruz, CABA, Argentina
Romina Gisele Praderio ; Animal Reproduction Research Institute (INIRA), Faculty of Veterinary Sciences, National University of La Plata, La Plata, Buenos Aires, Argentina; National Scientific and Technical Research Council (CONICET), Godoy Cruz, CABA, Argentina
María Cecilia Stornelli ; Animal Reproduction Research Institute (INIRA), Faculty of Veterinary Sciences, National University of La Plata, La Plata, Buenos Aires, Argentina
Rodolfo Luzbel De La Sota ; Animal Reproduction Research Institute (INIRA), Faculty of Veterinary Sciences, National University of La Plata, La Plata, Buenos Aires, Argentina; National Scientific and Technical Research Council (CONICET), Godoy Cruz, CABA, Argentina
María Alejandra Stornelli ; Animal Reproduction Research Institute (INIRA), Faculty of Veterinary Sciences, National University of La Plata, La Plata, Buenos Aires, Argentina *

* Corresponding author.

Abstract

Our study aimed to evaluate the gene transcription of Cyclooxygenase 1 (COX1), cytokines (Interleukin 6 [IL6], Interleukin 8 [IL8], and Leukemia Inhibitory Factor [LIF]), and Progesterone Receptor (PR) in clinically healthy bitches with a normal endometrium and with endometritis, without the presence of cystic endometrial hyperplasia. Forty-eight mixed breed bitches in dioestrus were used. Uterine biopsies were collected for diagnosis of a normal endometrium (n=15), and endometritis (n=30). Three samples were excluded from the study (two bitches with cystic endometrial hyperplasia and one bitch with atrophy). Samples were collected by cytobrush to quantify mRNA by qPCR. Data were analyzed using a generalized mixed model. COX1, IL6, and IL8 mRNA expression in bitches with endometritis was significantly higher than in a normal endometrium (P=0.0002, P=0.002, and P=0.003, respectively). In contrast, LIF and PR mRNA expression in bitches with endometritis was significantly lower than with a normal endometrium (P=0.006 and P=0.001, respectively). IL6 (AUC 0.67) was the most favorable biomolecular marker for predicting endometritis in bitches. There was an evident change in the mRNA expression of COX1, cytokines, and PR in clinically healthy bitches with a normal endometrium and endometritis without cystic endometrial hyperplasia. These results suggest that biomolecular markers such as COX1, IL6, IL8, LIF, and PR could help diagnose endometritis in a bitch.

Keywords

bitch; endometritis; cyclooxygenase 1; cytokines; progesterone receptor

Hrčak ID:

314177

URI

https://hrcak.srce.hr/314177

Publication date:

15.1.2024.

Article data in other languages: croatian

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