Acta clinica Croatica, Vol. 62. No. 3, 2023.
Original scientific paper
https://doi.org/10.20471/acc.2023.62.03.5
Does Gallic Acid Have a Potential Remedial Effect in Experimental Corrosive Burn Injury to the Esophagus?
Erol Basuguy
; Department of Pediatric Surgery, Faculty of Medicine, Dicle University, Diyarbakır, Turkey
*
Ebru Gokalp Ozkorkmaz
; Department of Histology and Embryology, Faculty of Medicine, Dicle University, Diyarbakır, Turkey
* Corresponding author.
Abstract
Gallic acid, acting as an antioxidant, anti-precipitant and cytoprotective agent, was
used as a possible remedial natural component for treating experimentally induced esophageal burn.
Wistar rats (n=24) were divided into three groups. Control group was given 1 mL 0.9% NaCl. Experimental
esophageal burn was induced with 1 mL 40% NaOH application to the esophagus in groups
2 and 3. Gallic acid® (20 mg/kg) was administered to the treated group via oral gavage for 10 days.
Removed tissues were fixed and paraffin blocks were prepared. Histopathological examination was
performed after the sections had been stained with hematoxylin-eosin. Tumor necrosis factor alpha
and caspase-3 antibodies were used on immunohistochemical analysis. In the esophageal burn group,
necrosis, degeneration and numerous apoptotic cells, as well as intense inflammatory cell infiltration
and fibrosis in the muscle layer were observed under light microscope. In the treated group, remodeling
of epithelial cells with marked reduction in the connective tissue collagen content was observed,
as well as marked reduction in the volume of collagen and abundance of inflammatory cells in blood
vessels. Gallic acid treatment may help heal esophageal burns and prevent complications.
Keywords
Esophageal burn; Gallic acid; Tumor necrosis factor alpha; Caspase-3; Immunohistochemistry
Hrčak ID:
314860
URI
Publication date:
1.11.2023.
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