Original scientific paper

https://doi.org/10.24099/vet.arhiv.2255

Analysis of genetic variations of a recombinant strain derived from the three lineages of porcine reproductive and respiratory syndrome virus-2

Limei Zheng ; Qingdao Agricultural University, Qingdao, China
Xiaoyan Wu ; Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Chang Liu ; Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Chen Li ; Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Jianli Shi ; Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Mengjie Huang ; College of Animal Science and Veterinary Medicine Shandong Agricultural University, Taiwan, China
Yingru Ma ; Qingdao Agricultural University, Qingdao, China
Hong Han ; Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Shaojian Xu ; Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Nataliia Hrabchenko ; Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Jianhua Qiu ; College of Animal Science and Veterinary Medicine Shandong Agricultural University, Taiwan, China
Jianxin Wen ; Qingdao Agricultural University, Qingdao, China
Jun Li ; Qingdao Agricultural University, Qingdao, China; Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Xianjie Han ; Qingdao Agricultural University, Qingdao, China *

* Corresponding author.

Abstract

Since the rise of the porcine reproductive and respiratory syndrome virus (PRRSV) in China, gene mutations have frequently occurred. To understand the current prevalence and evolution of PRRSV in Shandong Province, 1,528 samples suspected of PRRSV were collected from local pig farms of different sizes. The complete genome sequence of the PRRSV strain SDLY-27 was determined by next-generation sequencing (NGS) technology. The genomic sequence of SDLY-27 was 15,363 nucleotides (nt) in length, comparative analysis of the whole genome sequence suggested that the homology between SDLY 27 and 81 PRRSV strains from China and other countries in genbank was 61.9 ~ 96.4%. This study is the first to detect recombinants from multiple recombination events among the Lineage 8 (JXA1-like strains), Lineage 5 (RespPRRSV-MLV and VR2332 strains) and Sublineage 1.5 (NADC34-like strains) in Shandong, China, and provides new data for the epidemiological study of PRRSV in China. This study enriches the epidemiological data on PRRSV in Shandong Province, China. It provides an important reference for the development of new vaccines and for the prevention and control of PRRSV in China.

Keywords

PRRSV; recombination strains; GP5; NSP2; genetic evolutionary analysis; next-generation sequencing

Hrčak ID:

318359

URI

https://hrcak.srce.hr/318359

Publication date:

5.4.2024.

Article data in other languages: croatian

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