Original scientific paper

https://doi.org/10.24099/vet.arhiv.2255

Analiza genskih varijacija rekombinantnog soja dobivenog iz triju linija virusa-2 reproduktivnog i respiratornog sindroma svinja

Limei Zheng ; Qingdao Agricultural University, Qingdao, China
Xiaoyan Wu ; Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Chang Liu ; Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Chen Li ; Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Jianli Shi ; Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Mengjie Huang ; College of Animal Science and Veterinary Medicine Shandong Agricultural University, Taiwan, China
Yingru Ma ; Qingdao Agricultural University, Qingdao, China
Hong Han ; Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Shaojian Xu ; Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Nataliia Hrabchenko ; Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Jianhua Qiu ; College of Animal Science and Veterinary Medicine Shandong Agricultural University, Taiwan, China
Jianxin Wen ; Qingdao Agricultural University, Qingdao, China
Jun Li ; Qingdao Agricultural University, Qingdao, China; Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Xianjie Han ; Qingdao Agricultural University, Qingdao, China *

* Corresponding author.

Abstract

Usporedno sa širenjem virusa reproduktivnog i respiratornog sindroma svinja (PRRSV) u Kini, sve su češće bile i njegove genske mutacije. Kako bi se ustanovila trenutačna prevalencija i evolucija PRRSV-a u pokrajini Shandong, s lokalnih farmi prikupljeno je 1528 uzoraka svinja različitih kategorija za koje je postojala sumnja na zarazu PRRSVom. Kompletan genomski slijed soja SDLY-27 PRRSV-a određen je tehnologijom sekvenciranja sljedeće generacije (NGS). Slijed je imao dužinu od 15 363 nukleotida (nt), a komparativna analiza cijeloga genomskog slijeda uputila je na to da je homolognost između sojeva SDLY 27 i 81 PRRSV-a iz Kine i uzoraka u banci gena iz drugih zemalja 61,9~96,4%. Ovo je prvo istraživanje koje je otkrilo rekombinantne sojeve iz višestrukih rekombinacija među linijama 8 (sojevi nalik na JXA1), 5 (sojevi RespPRRSV-MLV i VR2332) i podlinije 1,5 (sojevi nalik na NADC34) u Shandongu, Kina.Kao takvo, istraživanje pruža nove podatke o epidemiologiji PRRSV-a u Kini, posebno u pokrajini Shandong, a ujedno predstavlja i važnu referenciju za razvoj novih cjepiva te prevenciju i kontrolu bolesti uzrokovane navedenim virusom.

Keywords

PRRSV; rekombinantni sojevi; GP5; NSP2; genetička evolucijska analiza; sekvenciranje sljedeće generacije

Hrčak ID:

318359

URI

https://hrcak.srce.hr/318359

Publication date:

5.4.2024.

Article data in other languages: english

Visits: 390 *