Original scientific paper

https://doi.org/10.5513/JCEA01/25.2.4305

Multispectral and chlorophyll fluorescence imaging for detection of nutrient deficiency symptoms in common bean

Boris LAZAREVIĆ ; Centre of Excellence for Biodiversity and Molecular Plant Breeding (CroP-BioDiv), Svetošimunska cesta 25, 10000 Zagreb, Croatia
Jerko GUNJAČA orcid.org/0000-0003-3999-4519 ; University of Zagreb Faculty of Agriculture, Department of Plant Breeding, Genetics and Biometrics, Svetošimunska cesta 25, 10000 Zagreb, Croatia
Toni SAFNER orcid.org/0000-0001-8645-5273 ; University of Zagreb Faculty of Agriculture, Department of Plant Breeding, Genetics and Biometrics, Svetošimunska cesta 25, 10000 Zagreb, Croatia
Monika VIDAK orcid.org/0000-0001-8022-4888 ; University of Zagreb Faculty of Agriculture, Department of Plant Biodiversity, Svetošimunska cesta 25, 10000 Zagreb, Croatia *
Tomislav JAVORNIK orcid.org/0009-0007-0595-1955 ; University of Zagreb Faculty of Agriculture, Department of Plant Biodiversity, Svetošimunska cesta 25, 10000 Zagreb, Croatia
Klaudija CAROVIĆ-STANKO orcid.org/0000-0002-8777-9875 ; University of Zagreb Faculty of Agriculture, Department of Plant Biodiversity, Svetošimunska cesta 25, 10000 Zagreb, Croatia

* Corresponding author.

Abstract

Crop production might suffer severe economic losses due to insufficient fertiliser availability. Specific signs of nutrient shortage influence plant morphology and physiology. This study pioneers the non-destructive tracking and characterization of nutrient deficiency symptoms in common beans using multispectral and chlorophyll fluorescence imaging, offering novel insights into the dynamic responses of plant morphology and physiology to specific nutrient shortages. Plants were cultivated in nutrient solutions with and without nitrogen (N), phosphorus (P), potassium (K), magnesium (Mg), iron (Fe) and control solution. Measurements were taken every three days for 12 days (MT1-MT4) of growth. K and N deficit plants had the earliest symptoms and most noticeable changes, whereas Fe deficiency plants had the slowest and least noticeable symptoms. Except for Fe, the most responsive chlorophyll fluorescence parameter was electron transport rate, which was reduced in plants from all nutrient deprivation treatments compared to control. All nutrient deficit treatments reduced leaf area at MT2, which was the most affected morphological parameter. The green leaf index, reflection in blue, and specific green were the most affected multispectral traits by nutritional deprivation. These findings demonstrate that plant nutrient deficit can be recognized and tracked non-destructively utilizing multispectral and chlorophyll fluorescence analysis. Overall, our work not only sheds light on the dynamics of nutrient deficiency in common bean plants but also offers practical implications for improving crop management strategies using non-destructive digital technology.

Keywords

image-based phenotyping, vegetation indices; plant nutrition; Phaseolus vulgaris

Hrčak ID:

318548

URI

https://hrcak.srce.hr/318548

Publication date:

28.6.2024.

Article data in other languages: croatian

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