Veterinarska stanica, Vol. 56 No. 2, 2025.
Original scientific paper
https://doi.org/10.46419/vs.56.2.3
Antimicrobial effectiveness of chestnut honey, pollen and propolis individually and in combination
Benjamin Čengić
; Faculty of Veterinary Medicine, University in Sarajevo, Bosnia and Herzegovina
*
Medina Rondić
; OS Miloslav Stikovic, Prijepolje, Serbia
Anesa Jerković-Mujkić
; Microbiology Laboratory, Department of Biology, Faculty of Science, University in Sarajevo, Bosnia and Herzegovina
Belmina Šarić-Medić
; Laboratory for Human Genetics, Institute for Genetic Engineering and Biotechnology, University in Sarajevo, Bosnia and Herzegovina
Amina Magoda
; Laboratory for Assessment of Residues and Food Control, Veterinary faculty, University in Sarajevo, Bosnia and Herzegovina
Amel Ćutuk
; Faculty of Veterinary Medicine, University in Sarajevo, Bosnia and Herzegovina
Pamela Bejdić
; Faculty of Veterinary Medicine, University in Sarajevo, Bosnia and Herzegovina
Sabina Šerić-Haračić
; Faculty of Veterinary Medicine, University in Sarajevo, Bosnia and Herzegovina
Alan Maksimović
; Faculty of Veterinary Medicine, University in Sarajevo, Bosnia and Herzegovina
* Corresponding author.
Abstract
The emergence of bacteria with antibiotic resistance and multiple resistance is characteristic of animal and human pathogens. It is wide known that bee products, which have been used in alternative medicine since ancient times, have antimicrobial potential. Application of bee products for therapeutic purposes is defined as apitherapy. The study aimed to evaluate the antimicrobial activity of commercial chestnut honey, pollen and propolis produced in western Bosnia and Herzegovina (Sanski Most) individually and in five combinations (apimixtures). The antimicrobial properties of samples were investigated using the agar well diffusion method against three Gram-positive bacteria (Bacillus subtilis subsp. spizizenii ATCC 6633, Methicillin-resistant Staphylococcus aureus ATCC 33591, Enterococcus faecalis ATCC 29212); three Gram-negative bacteria (ESBL producing Escherichia coli ATCC 35218, Salmonella enterica subsp. enterica serovar Enteritidis ATCC 13076, Pseudomonas aeruginosa ATCC 9027) and one fungal species (Candida albicans ATCC 10231). Pure bee pollen inhibited the growth of only Gram-negative bacteria, concentrated chestnut honey was active against all Gram-negative and Gram-positive
bacteria, while 20% propolis extract and apimixtures A2 (80% honey and 20% propolis) and A3 (60% honey, 20% pollen and 20% propolis extract) inhibited the growth of all tested microorganisms. Chestnut honey and
three apimixtures (A1, A2 and A3) showed the highest antibacterial action against all tested Gram-negative bacteria and MRSA compared to other investigated samples. In this study, examined honeybee products from Bosnia and Herzegovina and their mixtures had significant activity against tested bacteria, including strains with proven resistance to conventional antibiotics, MRSA and ESBL producing E. coli.
Keywords
bee products; apimixture; antibiotic; inhibition; resistance
Hrčak ID:
319594
URI
Publication date:
14.8.2024.
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