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Determination of 8-xydroxy-2’deoxyguanosine in Urine Using HPLC With Electrochemical Detection

Ana-Marija Domijan ; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Maja Peraica ; Institute for Medical Research and Occupational Health, Zagreb, Croatia

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page 277-282

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Endogenous cell metabolism and different chemicals, drugs, ionising radiation, solar light, cigarette smoking, and air pollution can induce oxidative damage to DNA. Oxidative DNA damage is involved in the development of different diseases, aging, and cancer. Urine 8-hydroxy-2’deoxyguanosine (8-oxodG) is widely accepted as a biomarker of the “whole body” oxidative DNA damage. The aim of this study was to introduce a method for determination 8-oxodG in spot urine samples using high-performance liquid chromatography with electrochemical detection. In the tested range of 50 nmol L-1 to 400 nmol L-1, the calibration curve was linear (r2=0.9995) and the limit of detection was 3 nmol L-1. Concentrations of 8-oxodG ranged between 3.0 nmol L-1 and 48.0 nmol L-1, which is in accordance with values reported for healthy individuals in other studies.


biomarker, free radicals, oxidative DNA damage, 8-oxodG

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