Original scientific paper

https://doi.org/https://doi.org/10.5599/jese.2478

Screen-printed electrode for electrochemical detection of sunitinib malate for therapeutic drug monitoring

Arun Warrier ; Department of Biotechnology, Sahrdaya College of Engineering & Technology, APJ Abdul Kalam Technological University Thiruvananthapuram, Kodakara, Thrissur, Kerala, India
Pooja Das Manjulabhai ; Department of Biotechnology, Sahrdaya College of Engineering & Technology, APJ Abdul Kalam Technological University Thiruvananthapuram, Kodakara, Thrissur, Kerala, India
Aiswarya Rajesh ; Department of Biotechnology, Sahrdaya College of Engineering & Technology, APJ Abdul Kalam Technological University Thiruvananthapuram, Kodakara, Thrissur, Kerala, India
Unnimaya Shanmughan ; Department of Biotechnology, Sahrdaya College of Engineering & Technology, APJ Abdul Kalam Technological University Thiruvananthapuram, Kodakara, Thrissur, Kerala, India
Varsha Vijayakumar ; Department of Biotechnology, Sahrdaya College of Engineering & Technology, APJ Abdul Kalam Technological University Thiruvananthapuram, Kodakara, Thrissur, Kerala, India
Jeethu Raveendran ; Department of Biomedical Engineering, Sahrdaya College of Engineering & Technology, APJ Abdul Kalam Technological University Thiruvananthapuram, Kodakara, Thrissur, Kerala, India
Dhanya Gangadharan ; Department of Biotechnology, Sahrdaya College of Engineering & Technology, APJ Abdul Kalam Technological University Thiruvananthapuram, Kodakara, Thrissur, Kerala, India

Abstract

Sunitinib is a targeted therapy for colorectal cancer, which needs a precise dosage due to potential severe side effects from overdose. Therapeutic drug monitoring is crucial for maintaining optimal drug levels in body fluids. Traditional anticancer drug dose evalua­tion methods such as high-pressure liquid chromatography, liquid chromatography with mass spectrophotometry, and immunoassays are cumbersome. This study explores the utilization of electrochemical sensors on indigenously developed screen-printed electrodes for sunitinib malate monitoring. Differential pulse voltammetry, cyclic voltam­metry and chrono-amperometric studies were conducted in a conventional three-elec­trode system. An anodic peak current, indicative of sunitinib malate electro­oxi­dation, was observed around +0.45 V vs. Ag/AgCl reference electrode in 0.1 M PB of pH 7.4. Sen­sitivity, the limit of detection and method detection limit were determined as 0.386 µA µM-1 cm-2, 0.009 and 0.0108 µM, respectively. The response exhibited linearity (R2 = 0.990) with sunitinib concentration ranging from 0.08 to 88 μM with good reproducibility. DPV studies on real samples yielded acceptable recovery values. Electro­chemical sensors based on the screen-printed electrode present a promising approach for sunitinib monitoring, offering sensitivity, low limit of detection, and a wide linear range. Since the expected plasma concentration of sunitinib is much higher than the sensor detection range, it can be used for real sample analysis. Here developed methods could simplify and improve therapeutic drug monitoring in colorectal cancer treatment.

Keywords

Anticancer drug; electrooxidation; differential pulse voltammetry; real samples; electrochemical sensor

Hrčak ID:

323685

URI

https://hrcak.srce.hr/323685

Publication date:

10.12.2024.

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