Original scientific paper
https://doi.org/https://doi.org/10.5513/JCEA01/25.4.4395
Effects of g.10329C>T polymorphism in the SCD gene with milk production traits in Bangladeshi cattle: evidence from mutant protein function analysis
Md Abdul ALIM
; Animal Biotechnology Division, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka-1349, Bangladesh
*
Mridha Mohammad Kamal HOSSAIN
; Animal Biotechnology Division, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka-1349, Bangladesh
Nusrat JAHAN
; Animal Biotechnology Division, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka-1349, Bangladesh
Rubaya RUBAYA
; Animal Biotechnology Division, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka-1349, Bangladesh
Anjuman Ara BHUYAN
; Animal Biotechnology Division, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka-1349, Bangladesh
Jahangir ALAM
; Animal Biotechnology Division, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka-1349, Bangladesh
Ishtiaque AHAMMAD
; Bioinformatics Division, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka-1349, Bangladesh
Arittra BHATTACHARJEE
; Bioinformatics Division, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka-1349, Bangladesh
Mohammad Shohel Rana SIDDIK
; Department of Dairy Science, Bangladesh Agricultural University, Mymensingh -2202, Bangladesh
Md MONIRUZZAMAN
; Molecular Biotechnology Division, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dha-ka-1349, Bangladesh
* Corresponding author.
Abstract
This study aimed to pinpoint the single nucleotide polymorphisms (SNPs) within the stearoyl-CoA desaturase (SCD) gene that is notably linked to milk production traits and to evaluate the structural and functional consequences of mutant proteins. Through pooled DNA sequencing, five SNPs were identified within the SCD gene. Among these, only one exonic SNP (g.10329C>T) was non-synonymous, resulting in an amino acid change from alanine to valine in the protein. Structural and functional analysis of the g.10329C>T mutation revealed discernible differences between wildtype and mutant proteins, indicating consequential effects on the observed phenotype. Subsequently, this identified SNP was genotyped in 100 milking cows for association analysis. The SNP g.10329C>T demonstrated significant allele substitution effects on milk yield traits. These findings suggest that the identified polymorphism (g.10329C>T) influences milk yield traits within the studied population and could serve as a genetic marker for cattle selection processes aimed at enhancing productivity with proper validation.
Keywords
Single nucleotide polymorphism; association; SCD; mutant proteins
Hrčak ID:
324552
URI
Publication date:
23.12.2024.
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