Original scientific paper

https://doi.org/https://doi.org/10.5513/JCEA01/25.4.4233

Characteristics of Slovak amaranth varieties based on coding regions polymorphism and endophytic bacteria prevalence

Dagmar MORAVČÍKOVÁ ; Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Institute of Plant and Environmental Sciences; Tr. A. Hlinku 2, 94976, Nitra, Slovak Republic
Matúš KYSEĹ ; Department of Dendrobiology, Institute of Forest Ecology, Slovak Academy of Sciences - Zvolen, Vieska nad Žitavou 178, 951 52 Slepčany, Slovak Republic
Silvia FARKASOVÁ ; Slovak University of Agriculture in Nitra, Research Centre of AgroBioTech; Tr. A. Hlinku 2, 94976, Nitra, Slovak Republic
Andrea HRICOVÁ ; Slovak Academy of Sciences, Centre of Plant Biology and Biotechnology, Institute of Plant Genomic and Biology, Akademická 969, 949 01, Nitra, Slovak Republic
Veronika ŠTEFÚNOVÁ ; Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Institute of Plant and Environmental Sciences; Tr. A. Hlinku 2, 94976, Nitra, Slovak Republic
Jana ŽIAROVSKÁ ; Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Institute of Plant and Environmental Sciences; Tr. A. Hlinku 2, 94976, Nitra, Slovak Republic *

* Corresponding author.

Abstract

Amaranthus is one of the underutilised crops with the potential to overcome some of the current challenges of modern agriculture under highly unstable environmental conditions. Here, two Slovak varieties of amaranth – Zobor (A. hypochondriacus × A. hybridus) and Pribina (A. cruentus) were characterised by Conserved DNA-Derived Polymorphism (CDDP), P450-based analogue (PBA) markers and Tubulin-Based Polymorphism (TBP) and by its most abundant endophytes. CDDP and TBP profiles showed a very similar distribution of the generated fragments for both of the analyzed varieties. PBA profiles generated the lowest value of polymorphism. A wide range of amplicons was generated by all of the techniques used in the study with the length of PCR fragments ranging from 80 up to 2292 bp. The variability of the endophytic composition of the Pribina variety showed the following three most abundant genera: Endobacter, Dichotomicrobium and Burkholderia. Abiotrophia, Endobacter and Limimonas were most abundant in the Zobor variety.

Keywords

Amaranthus; Zobor variety; Pribina variety; coding regions markers; endophytes

Hrčak ID:

324747

URI

https://hrcak.srce.hr/324747

Publication date:

23.12.2024.

Article data in other languages: slovak

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