Original scientific paper

Improved Performances of Polyacrylamide Gel by Addition of a New Type of Polymer: Detection of Allelic Losses

Marina Korolija ; Ruđer Bošković Institute, Zagreb, Croatia
Mirko Hadžija ; Ruđer Bošković Institute, Zagreb, Croatia
Pajica Pavković ; Vuk Vrhovac University Clinic, Zagreb, Croatia
Sanja Kapitanović ; Ruđer Bošković Institute, Zagreb, Croatia
Marijana Popović Hadžija orcid.org/0000-0001-6522-3721 ; Ruđer Bošković Institute, Zagreb, Croatia

Abstract

The aim of this study was to improve detection of heterozygous samples and loss of heterozygosity
using a new type of gel polymer. A total of 60 samples of normal and tumor colon tissue
were tested. Elctrophoresis of amplified loci was performed on a standard acrylamide / N,Nmethylenebisacrylamide
gel, and the same gel containing Spreadex polymer, NAB (native acrylamide-
bis). Standard gel displayed 36 samples of normal tissue as homozygous. Electrophoresis
of the same samples on gel with addition of polymer revealed 12/36 heterozygous samples.
Five of 12 matched tumor samples displayed loss of heterozygosity that could not be detected
on standard gel, because of its lower resolving power. Electrophoresis on a new polymer presents
a simple, inexpensive, reproducible and non-radioactive method for loss of heterozygosity
detection, with improved performances such as increased sensitivity, decreased amount of a
sample and improved separation of closely spaced bands. Application of the polymer may contribute
to a vide variety of diagnostic procedures.

Keywords

DPC4; loss of heterozygosity; allele; Spreadex polymer NAB

Hrčak ID:

31128

URI

https://hrcak.srce.hr/31128

Publication date:

15.11.2008.

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