Original scientific paper

Potentiometric Investigation of Specific Ionic Effects on Interactions between Bovine Serum Albumin and Weak Polyelectrolytes

Sanela Mutka ; Laboratory of Physical Chemistry, Department of Chemistry, Faculty of Science, University of Zagreb, Zagreb, Croatia
Branka Njegić-Džakula ; Laboratory for Precipitation Processes, Ruđer Bošković Institute, Zagreb, Croatia
Davor Kovačević orcid.org/0000-0001-6446-3459 ; Laboratory of Physical Chemistry, Department of Chemistry, Faculty of Science, University of Zagreb, Zagreb, Croatia

Abstract

The effect of salt on the behaviour of bovine serum albumin (BSA) in solution and on the interactions
between BSA and the weakly charged polyelectrolytes poly(allylamine hydrochloride)
and poly(dimethylaminoethylmethacrylate) was investigated by potentiometric titrations. Titrations
of pure BSA solution and of the BSA solution with the addition of polyelectrolyte were
performed in the presence of different salts. Three electrolytes having the same anion and a different
cation were used, lithium chloride, sodium chloride, and caesium chloride. The corresponding
base served as titrant. Experiments were performed at four electrolyte concentrations.
The titration curves showed a strong influence of ionic strength and the electrolyte type. The
cation effect was found to be in accordance with the Hofmeister series.

Keywords

potentiometric titration; bovine serum albumin; weak polyelectrolytes; ionic strength

Hrčak ID:

31184

URI

https://hrcak.srce.hr/31184

Publication date:

31.12.2008.

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