Original scientific paper

Effect of Indole-3-butyric Acid on Rooting of Phytoplasma-recovered and Healthy Periwinkle Catharanthus roseus (L.) G. Don

Mirna Ćurković-Perica ; University of Zagreb, Faculty of Science, Division of Biology, Department of Botany, Zagreb, Croatia

Abstract

After prolonged treatment with the auxin, indole-3-butyric acid (IBA), the rooting efficiency of
healthy C. roseus shoots was compared with recovered shoots containing a high titer of
'Candidatus Phytoplasma' species 'Ca. P. ulmi' and 'Ca. P. solani' and recovered shoots in
which, upon IBA-treatment, 'Ca. P. asteris' was undetectable or present in low titer. On a medium
containing the same concentration of IBA, the number of rooted shoots decreased with
statistical significance, following the trend: healthy shoots > 'Ca. P. asteris'-recovered shoots >
'Ca. P. ulmi' and 'Ca. P. solani'-recovered shoots. Furthermore, after the rooting experiment,
'Ca. P. asteris' was detected in all the tested unrooted shoots while, among the rooted periwinkles,
only 20 % were positive. These results constitute the first report in which a correlation
between the presence of phytoplasmas and rooting efficiency of the periwinkle host has been
shown. Since auxins are phytohormones involved in the initiation and emergence of adventitious
roots, reduced rooting efficiency may reflect disturbed levels or transport of auxin(s) in
phytoplasma-infected periwinkles.

Keywords

bacteria; Catharanthus roseus; indole-3-butyric acid; phytoplasma; rooting; tissue culture

Hrčak ID:

31191

URI

https://hrcak.srce.hr/31191

Publication date:

31.12.2008.

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