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Natura Croatica : Periodicum Musei Historiae Naturalis Croatici, Vol. 17 No. 4, 2008.

Original scientific paper

Elektrokemijsko određivanje metalotioneina kod domaće peradi

Miroslava Beklova ; Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, CZ-612 42 Brno, Czech Republic
Ivo Fabrik ; Department of Chemistry and Biochemistry Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic
Pavlina Sobrova ; Department of Chemistry and Biochemistry Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic
Vojtech Adam ; Department of Chemistry and Biochemistry; 3Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic
Jiri Pikula ; Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, CZ-612 42 Brno, Czech Republic
Rene Kizek ; Department of Chemistry and Biochemistry Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic

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Abstract

Metalotionein (MT) pripada grupi intracelularnih proteina male molekularne mase bogatih cisteinom, s molekularnom masom od 6 do 10 kDa. Zbog njihovog afiniteta prema teškim metalima (Zn, Cd, As, itd.) njihova glavna uloga je homeostatska kontrola i detoksifikacija iona metala u organizmu. U ovom radu predlažu se elektroanalitičke tehnike za određivanje sadržaja MT u krvnom serumu domaće peradi. Elektrokemijska mjerenja izvršena su uređajem AUTOLAB Analyser povezanim s VA-Stand 663, koristeći standardnu ćeliju s tri elektrode. Osim toga MT je određivan tehnikom adsorptivnog transfera, povezanoj s voltmetrijom diferencijalnog pulsa. Granica detekcije MT je procjenjivana do 100 fM (samo standardi) ili do 100 pM, mjereno u prisutnosti krvnog seruma. Prosječni sadržaj MT bio je 21.3 µM. Razina MT kod kokoši bila je otprilike 25% viša nego kod pijetlova. Ta pojava može se objasniti većom potrebom za ovim proteinom kod kokoši zbog transporta iona prilikom stvaranja ljuske jajeta.

Keywords

elektrokemijska metoda; voltmetrija diferencijalnog pulsa; Brdicka-reakcija; protein; ptice

Hrčak ID:

34029

URI

https://hrcak.srce.hr/34029

Publication date:

31.12.2008.

Article data in other languages: english

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