Original scientific paper

Amperometric Adhesion Signals of Liposomes, Cells and Droplets

N. Ivošević DeNardis ; Laboratory for Bioelectrochemistry and Surface Imaging, Division for Marine and Environmental Research, Ruđer Bošković Institute, Bijenička 54, 10 000 Zagreb, Croatia
V. Žutić ; Laboratory for Bioelectrochemistry and Surface Imaging, Division for Marine and Environmental Research, Ruđer Bošković Institute, Bijenička 54, 10 000 Zagreb, Croatia
V. Svetličić ; Laboratory for Bioelectrochemistry and Surface Imaging, Division for Marine and Environmental Research, Ruđer Bošković Institute, Bijenička 54, 10 000 Zagreb, Croatia
R. Frkanec ; Institute of Immunology, Inc., PO Box 266, 10 000 Zagreb, Croatia

Abstract

Individual soft microparticles (liposomes, living cells and organic droplets) in aqueous media are characterized by their adhesion signals using amperometry at the dropping mercury electrode. We confirmed that the general mechanism established for adhesion of
hydrocarbon droplets and cells is valid as well for liposome adhesion within a wide range of surface charge densities. Incidents and shape of adhesion signals in liposome suspensions reflect liposome polydispersity, surface charge density and properties of phospholipid head group. Major distinction in adhesion behavior of liposomes when
compared to organic droplets was identified as: (i) different values of critical interfacial tensions of adhesion at the positively and the negatively charged electrode, and (ii) appearance of signals revealing the specific interactions of phospholipid polar head groups
with the electrode.

Keywords

Amperometric adhesion signals; DOPC liposomes; dropping mercury electrode; microparticles; PS liposomes

Hrčak ID:

34611

URI

https://hrcak.srce.hr/34611

Publication date:

27.3.2009.

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