Original scientific paper
https://doi.org/10.2478/10004-1254-60-2009-1891
Disturbance of Cell Proliferation in Response to Mobile Phone Frequency Radiation
Ivančica Trošić
; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Ivan Pavičić
; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Abstract
The aim of study was to determine the infl uence of mobile phone frequency radiation on the proliferation, cytoskeleton structure, and mitotic index of V79 cells after 1 h, 2 h, and 3 h of exposure. V79 cells were cultured in standard laboratory conditions and exposed to continuous-wave (CW) RF/MW radiation of 935 MHz, electric fi eld strength of (8.2±0.3) V m-1, and specifi c absorption rate (SAR) of 0.12 W kg-1. To identify proliferation kinetics, the cells were counted for each hour of exposure 24 h, 48 h, 72 h, and 96 h after respective exposures. Microtubule proteins were determined using specifi c immunocytochemical methods. Cell smears were analysed under a fl uorescent microscope. The study included negative and positive controls. Mitotic index was determined by estimating the number of dividing cells 24 h after exposure and dividing it with the total number of cells. In comparison to the controls, cell proliferation declined in cells exposed for three hours 72 h after irradiation (p<0.05). Microtubule structure was clearly altered immediately after three hours of irradiation (p<0.05). The mitotic index in RF/MW-exposed cells did not differ from negative controls. However, even if exposure did not affect the number of dividing cells, it may have slowed down cell division kinetics as a consequence of microtubule impairment immediately after exposure.
Keywords
continuous cell culture; microtubule structure; mitotic index; RF/MW; 935 MHz
Hrčak ID:
34876
URI
Publication date:
27.3.2009.
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