Original scientific paper

Ultra rapid open pulled straw (OPS) vitrification is a perspective for freezing horse embryos

Juraj Grizelj orcid.org/0000-0001-5963-3409 ; Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zagreb, Croatia
Guy Duchamp ; INRA , UMR0085, UMR INRA /CNRS /Haras Nationaux/Univ. Tours: Physiologie de la Reproduction et des Comportements, Physiologie Animale et Systèmes d’Elevage, Centre de Recherche de Tours, NOUZILLY, France
Florence Guignot ; INRA , UMR0085, UMR INRA /CNRS /Haras Nationaux/Univ. Tours: Physiologie de la Reproduction et des Comportements, Physiologie Animale et Systèmes d’Elevage, Centre de Recherche de Tours, NOUZILLY, France
Marianne Vidament ; INRA , UMR0085, UMR INRA /CNRS /Haras Nationaux/Univ. Tours: Physiologie de la Reproduction et des Comportements, Physiologie Animale et Systèmes d’Elevage, Centre de Recherche de Tours, NOUZILLY, France
Aurelie Plotto ; INRA , UMR0085, UMR INRA /CNRS /Haras Nationaux/Univ. Tours: Physiologie de la Reproduction et des Comportements, Physiologie Animale et Systèmes d’Elevage, Centre de Recherche de Tours, NOUZILLY, France
Pascal Mermillod ; INRA , UMR0085, UMR INRA /CNRS /Haras Nationaux/Univ. Tours: Physiologie de la Reproduction et des Comportements, Physiologie Animale et Systèmes d’Elevage, Centre de Recherche de Tours, NOUZILLY, France

Abstract

The aim of this research was to evaluate ultra rapid OPS vitrification on the embryo viability. The OPS vitrification technique is comprised of ultra rapid freezing of a small drop in which the embryo is placed. Before the thin straw was plunged into the liquid nitrogen, the embryos were treated with highly concentrated cryoprotectant (CPA) solutions as follows: 18% ethylene glycol (EG), 18% dimethyl-sulfoxide (DMSO) and 0.4 M sucrose. Surgical transfer into the recipient mares and morphologic examination of recollected embryos were used to measure the viability of transferred embryos. The research was performed on Welsh pony mares by collecting the embryos 6.75 days after ovulation. Twenty embryos were vitrified and transferred, four in each recipient mare. At day twelve, nine embryos were recollected after fl ushing of the recipient uterus (56%, 9/ 16). In one recipient mare, endometritis was detected when the uterus was fl ushed. Among the sixteen recollected embryos, seven (44%) had normal morphology and well developed embryonic vesicles. The vitrification procedure used proved to be encouraging.

Keywords

horse; embryo; cryopreservation; open pulled straw; vitrification; transfer

Hrčak ID:

36259

URI

https://hrcak.srce.hr/36259

Publication date:

20.4.2009.

Article data in other languages: croatian

Visits: 2.041 *