Meeting abstract
Evaluation of Bone Remodelling After Endodontic Therapy of the Periapecal Lesion
D. Kuiš
M. Kovačević
T. Tamarut
S. Bešlić
D. Šnjarić
J. Horvat
Abstract
The aim of the study was to evaluate bone remodelling by measuring osteoid surface, osteoid thickness, osteoclast index and inflammatory cell count after two differnet approaches in endodontic therapy of periapecal lesion on dogs’ teeth.
After inducing periapecal lesion in 23 mandibular premolars (37 root canals) from six mongrel dogs, 17 root canals were instrumented to the apical delta with crowndown techniwue using ProFile® Ni-Ti rotary instruments and filled to the same length with Thermafill® obturation technique and Top Seal® cement (group 1). The other 20 root canals (group 2) were instrumented using controlled overinstrumentation technique to the point determined ellectronically (electronic apex locator EED-11, Struja, Zagreb, Croatia). Teeth were obturated with Thermafill® at the length 2mm shorter than the working length. Length of obturation was confirmed radiographically and access cavities sealed with amalgam in both groups. The animals were sacrified 35 days after the end of endodontic treatment. Undemineralizad sections 5-7 mm thick were stained with toluidine blue. The histomorphometric indices (oseoid surface, osteoid thickness, osteoclast index and inflammatory cell count) were measured by light microscopy using computer program (ISSA, Vams, Zagreb, Croatia).
The difference between grops was statistically significant in all measured indices. In group 1 the osteoid surface was lower (10.34 % ± 11.60) as well as osteiod thickness (15.62 µm ± 7.41) and inflammatory cell count (111.39 ± 75.81) while osteoclast index was higher (111.34 mm - 2 ± 115.46). In group 2 osteoid surface was higher (33.21 % ± 21.43) as well as osteoid thickness (16.26 µm ± 6.46) and inflammatory cell count (137.62 ± 46.34), while osteoclast index was lower (27.00 mm - 2 ± 39.03). Stastistical analysis was performed using Menn-Whitney U Test.
Results obtained by measuring histomorphometric indices indicate sustained bone resorption activity in group 1 anf faster bone formation in group 2. The greater number of inflammatory cells in group 2 was probaly caused by additional trauma of periapical tissues during overinstrumentation. This trauma does not interfere with bone remodelling.
Keywords
Hrčak ID:
3418
URI
Publication date:
15.9.2003.
Visits: 1.528 *